Impact of Culture Media and Temperature on Growth and Sclerotial Formation of Macrophomina phaseolina (Tassi) Goid. causing dry Root Rot of Mothbean

Author: Megha Jaimini, J.R. Verma, Dama Ram, Surjeet and Govind Junjadia

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Abstract

Macrophomina phaseolina causal agent of dry root rot and charcoal rot in various pulses crops. Due to fluctuation of temperature and climatic disturbance in arid region is quite difficult to manage the dry root rot. To reduce the number of sclerotia in soil or to minimize the contact of the inoculums and the host, six different culture media and temperature were tested for their suitability on mycelial growth and sclerotial formation of the M. phaseolina in vitro conditions. Among the six media PDA recorded highest (90.00 mm) followed by Richard’s agar (86.75 mm) media best for mycelial growth as well as for sclerotial formation. While, minimum growth (43.00 mm) was recorded in Sabouraud’s agar medium. Six different temperature levels viz., 15, 20, 25, 30, 35 and 40ºC were used for the study. The results indicated that the mycelial growth of M. Phaseolina was significantly grew best at 30ºC temperature (90.00 mm) followed by 35ºC temperature (84.50 mm), which was reduced significantly below 15°C and above 40°C.

Keywords

Mothbean, media, temperature, sclerotia, PDA, Macrophomina phaseolina

Conclusion

Overall the pathogen associated with dry root rot of mothbean was grow best on Potato dextrose agar media as well as excellent sclerotial formation on PDA at 30ºC temperature which was reduced significantly below 20°C and above 35°C in artificially conditions.

References

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How to cite this article

Megha Jaimini, J.R. Verma, Dama Ram, Surjeet and Govind Junjadia (2023). Impact of Culture Media and Temperature on Growth and Sclerotial Formation of Macrophomina phaseolina (Tassi) Goid. causing dry Root Rot of Mothbean. Biological Forum – An International Journal, 15(10): 679-683.