INTRODUCTION The grape (Vitis vinifera L.) is a fruit crop that belongs to the family Vitaceae, which is related to the northern temperate zone. It is one of the most commercially vital crops in the world. The grape is a woody vine in nature and can grow up to 17m or more. The fruits that plants produce is known as grapes (Kaur, 2017). Grapes contain a very good number of vitamins, minerals and other beneficial elements such as Ca, P, K and sugar. These sugars are basically present in the form of glucose and fructose. The amount of sugar content in grapes varies according to the variety. Grapes are used for various purposes such as for salad, jam & jelly and vinegar preparation. The domesticated type of grape is mostly utilized for selling purposes either for the production of processed products or for wine preparation. European grapes are considered the best ones for raisins, which are categorized according to size, shape and grape color. If we talk about its cultivation, India comes in the first ten countries that produce grapes (Wang et al., 2011). The global production of grapes is estimated at 78,034,332 metric tonnes (FAO, 2020). China, Italy, the USA, France, Spain, Turkey and Argentina are the countries that are the major producers of grapes globally. The leading country is China, with the production of 14,769,088 tonnes (FAO, 2020). In India, production of grapes is 31, 25, 000 MT (FAO, 2020). In India, among all fruits, grapes come in the 7th position and contribute up to 2% of the total production of grapes worldwide (Kaur, 2017). In India, 80% of production is given by Maharashtra and after that by Karnataka and Tamil Nadu. Grapes are one of the flexible crops which can tolerate any type of climate, but the climate of the Mediterranean region is most suitable for its production. In Punjab, around 777 hectares of land are used to cultivate grapes. There are several varieties that have been released and found suitable for Punjab conditions and give maximum productivity, such as Punjab MACS Purple, Perlette, Beauty seedless, etc. To fulfill the research gap, shoot and leaves initiation in grape cutting can be achieved by using various hormones and other treatments, including cinnamon powder, aloe vera, apple cider and honey, because they are the cheapest way to propagate and enhance the rooting quality. These growth hormones are easy to use, sustainable and cost effective. By incorporating these, it makes the soil more productive and, as a natural substance, it increases the growth and shoot of cuttings so increase their fresh and dry weight. Cinnamon is a kind of spice that belongs to the Lauraceae family. It is made from the bark of a cinnamon tree. It is mostly used as an aromatic condiment for culinary purposes, especially to enhance the flavor of dishes. It contains the essential oil component cinnamaldehyde along with eugenol, which gives a specific aroma to the dishes. Although it is used as a natural hormone, it also acts as a natural antibacterial and antimicrobial agent (Rajan et al., 2021). MATERIALS AND METHOD The current study was conducted in the agriculture research farm of Lovely Professional University, Punjab during the year of 2021-2022, to assess the Effect of natural and synthetic hormones on rooting of grape cutting (Vitis vinifera L) cv. Punjab Macs Purple. For this experiment, different types of natural and synthetic hormones were used. Materials and methods used in the present experiment have been illustrated in this segment. Disease-free and healthy, seedlings of grapes variety i.e., Punjab Mac Purple were obtained from the Regional Research Station, Bathinda (Punjab). This variety of grapes is commonly used by local farmers. The aloe vera, cinnamon powder, apple cider, honey, IAA, IBA and GA3 (Fig. 1) used in the experiment. Cuttings were quickly planted in plant trainers (after treatment) at 7.5cm deep up to the surface within rooting media. Studies are also operated upon to observe the effect of natural and synthetic growth hormones on rooting of grape cutting e.g. number of leaves, leaf length, fresh weight and dry weight. Find that which concentration or plant growth regulator is responsible for good shooting and others. Method of preparation of solution: T9 - IBA 2000 ppm (2 min) – Two gram of balanced IBA was taken in 1000 ml beaker and 1 to 2 drops of ethyl alcohol was added to dissolve the IBA properly. Slowly added distilled water with the help of glass rod IBA was dissolved to make final volume of 1 liter. T1 - Aloe Vera 100% – 1. Harvest aloe vera leaf (2kg for 500ml) after selecting healthy plant. 2. Extract aloe vera gel.a. Cut at harvested point using sharp knife. Keep this part in a beaker. Gel was extracted by cutting the cuticle with a knife. Cuticle was then grinded and added to the beaker. The green rind or cuticle of aloe vera plant and aloe vera outer leaf pulp contains latex was peeled with knife, grinded and put it in the beaker. c. Collect aloe vera gel and put in a beaker. Mix these three parts inside a beaker. Kept it in a refrigerator or kept outside after wrapping with aluminium foil. Kept in a cool place where the sunlight was not directly falling on it.3. Take 100 ml of aloe vera gel in a container or beaker at the time of treatment. T14 - Control - dipping in distilled water – Distilled water was taken in 500 ml beaker. The observations were recorded at three stages such as 30 DAPS, 60 DAP and 90 DAP. The recorded observations of morphological and biochemical parameters and the standard procedure adopted during the course of study are given Plate 1. RESULT AND DISCUSSION Number of leaves (no.). Data regards to the impact of natural and synthetic growth hormones on number of leaves, and survival percentage of cuttings was showed in table 1. The data on number of leaves was recorded and showed that the treatment T9 with the application of 2000 ppm IBA shows a maximum no. of leaves (17.33) followed by the treatment T1 which is the application 100% aloe vera with (16). The least no. of leaves was found in the treatment T3 which is the application of 100% apple cider with (5.00) at 30DAP. At 60DAP, the maximum no. of leaves (28.66) was found in the treatment T9 with the application of 2000 ppm IBA followed by the treatment T1 (26.66) with the application of 100% aloe vera. The least no. of leaves was found in the treatment T3 which is the application of 100% apple cider with (7.33). At, 90DAP, the maximum no. of leaves was found in the treatment T9 (118.33) with the application of 2000 ppm IBA followed by the treatment T1 (112.33) with the application of 100% aloe vera as compared to control (13.33). The number of leaves increased in treatment T9 (2000 ppm IBA) might be due to the in development of root IBA plays an important role and automatically this effect is shown in leaf length and leaf number and an increase in number of shoots due to IBA application may necessitate the increased activity of photosynthesis, transpiration and respiration in leaves and thus may result in the increase in number of leaves this result was supported by Jan et al. (2015); Wahab et al. (2001); Muttulani et al., (2022). Leaf length (cm). Data on leaf length is presented in Table 2 indicating that the treatments significantly control the leaf length over the control. Treatment T9 with the application of 2000 ppm IBA shows maximum leaf length (4.20 cm) followed by the treatment T1 (Aloe vera, 4 cm). Moreover, the least leaf length (2.36 cm) was found in the treatment T3 (Apple Cider) at 30DAP. At 60DAP, the maximum leaf length (4.43 cm) was found in the treatment T9 with the application of 2000 ppm IBA followed by the treatment T1 (Aloe vera, 4.26cm). Moreover, the least leaf length (2.00 cm) was found in the treatment T3 (Apple Cider). At, 90DAP, the maximum leaf length (7.53 cm) was found in the treatment T9 with the application of 2000 ppm IBA followed by the treatment T1 (Aloe vera, 6.03cm). Moreover, the least leaf length (2.36 cm) was found in the treatment T3 (Apple Cider). An increasing trend in length of leaves were observed from 30, 60 and 90 DAP respectively. The length of leaves increased in treatment T9 (2000 ppm IBA) might be due to the development of root IBA plays an important role and automatically this effect is shown in leaf length and leaf number and an increase in number of shoots due to IBA application may necessitate the increased activity of photosynthesis, transpiration and respiration in leaves and thus may result in the increase in length of leaves by (Wahab et al., 2001) in Guava. Similar results also recorded by (Jan et al., 2015) in olive cuttings. Length of leaves also increased in Aloe Vera gel treated grape cuttings, it might be due to Aloe Vera contains very essential number of macronutrients, micronutrients, vitamins, gibberellins and salicylic acid and has a stimulating effect on plant growth and development. Aloe Vera gel contains IAA which could be the alternative root hormone (Uddin et al., 2020). Similar results also recorded by (Hamouda et al., 2012). Fresh weight and Dry weight (gm). Data on fresh weight is presented in Table 3 indicated that the treatments significantly control the fresh weight over the control. The data on fresh weight showed that the maximum fresh weight was observed in the treatment T9 which is the application of 2000 ppm IBA with (52 gm) followed by the treatment T1 which is the application of 100% of aloe vera juice with 45.33 gm. The least fresh weight was found in the treatment T3 with the application of 100% apple cider vinegar with 10.66 gm as compared to control. This result was supported by Galavi et al. (2013) as they stated that fresh weight was increased by the application of IBA @400g/ml. Data on dry weight is presented in Table 3 indicated that the treatments significantly control the dry weight over the control. The data on dry weight showed that the maximum dry weight was observed in the treatment T9 which is the application of 2000 ppm IBA with (6.02 gm) followed by the treatment T1 which is the application of 100% of aloe vera juice with 5.30 gm. The least dry weight was found in the treatment T8 with the application of 1000 ppm IBA with 2.30 gm as compared to control. This result was supported by Galavi et al. (2013) as they stated that fresh weight was increased by the application of IBA @600g/ml.