The present study deals with the importance of phytochemical constituents of Hemionitis arifolia in treating breast cancer. This plant has many medicinal properties, such as antibacterial, hypoglycemic, antifungal, anti-inflammatory, antioxidant, and potent anti-cancer activities. Much research is to be done to know the mechanism involved. It has a very good healing property. We aimed to know the potential of the phytochemical constituents from Hemionitis arifolia against the breast cancer proteins. The two phytochemical constituents from Hemionitis arifolia topotecan, macdougallin, and the standard drug (Camptothecin) were used in this study. Based on the results obtained from Molecular docking analysis, all these phytochemical constituents have the best binding affinity towards breast cancer proteins EGFR and ER-α. From this, we can know the plant's usage and research in Hemionitis arifolia helps in the possibility of new drug development to treat breast cancer

Hemionitis arifolia, Camptothecin, Macdougallin, Topotecan, Molecular docking, ER-α, EGFR, Breast cancer

From the present study, it was concluded that the bioactive compounds identified from Hemionitis arifolia, i.e., especially topotecan and macdougallin, exhibited stronger binding affinities to breast cancer proteins compared to the standard drug, camptothecin. This in silico analysis provides valuable insights into the identification of the best phytochemical constituents. Future research will focus on isolating these compounds from Hemionitis arifolia and then performing in vitro analyses to further evaluate their therapeutic potential

Sashikanth Reddy G., Durga R., Praveen Kumar K. and Suneetha Y. (2025). In-silico Analysis of Phytochemical Constituents from Hemionitis arifolia Against Breast Cancer Proteins. Biological Forum, 17(4): 79-84