In vitro Analysis of Seedling Parameters in Groundnut under Induced EMS Mutagenesis

Author: Yugandhar A., K. Srinivas Naik, Bhattu Rajesh Nayak, Kumara Joshi and G. Vijay Kumar

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Abstract

The present in vitro analysis of seedling parameters of groundnut was carried out with three genotypes (ICG2106, ICG5236 and ICG76) at Department of Genetics, Osmania University, Hyderabad. The main aim of the present work is to observe the impact of seed germination and seedling traits under induced EMS in groundnut. The seeds were treated with three different concentrations of EMS viz., 0.3%, 0.4%, and 0.5%, and the control, and kept in petri plates. The germination (%) was measured on the seventh day following treatment, while the shoot, root length and number of leaves of the seedlings were measured on the fifteenth day. The ANOVA revealed that significant differences between treatments for all three traits under study. The results also revealed that ICG76 showed highest seed germination at 0.4% and ICG 5236 recorded highest shoot length, root length and number of leaves at 0.3% EMS concentration in our study.

Keywords

Groundnut, EMS, germination% and seedling traits

Conclusion

The results can be concluded that significant variability were observed for shoot length, root length, and number of leaves among three genotypes under various EMS concentration. The results also concluded that among three genotypes ICG76 showed highest seed germination at 0.4% and ICG 5236 recorded highest shoot length, root length and number of leaves at 0.3% EMS concentration.

References

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How to cite this article

Yugandhar A., K. Srinivas Naik, Bhattu Rajesh Nayak, Kumara Joshi and G. Vijay Kumar (2023). In vitro analysis of seedling parameters in groundnut under induced EMS mutagenesis. Biological Forum – An International Journal, 15(10): 393-397.