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In vitro and In Silico Evaluation of Anti-Microbial Potential of Leptadenia reticulata - An Endangered Medicinal Plant

Author: Sudipta Kumar Mohanty, Indu B.K., Jesse S. Dondapati and Anuradha M.

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Abstract

The present study reports the antimicrobial efficacy of bioactive compounds present in Leptadenia reticulata. The ethyl acetate extract shows highest antibacterial activity against E. coli. with 16.6 mm zone of inhibition. The methanol extract showed moderate activity against bacteria but produced highest zone of inhibition (24.8 mm, 23.6 mm) against Aspergillus niger and Trichoderma viride. The strong antibacterial activity of ethyl acetate extract against Staphylococcus aureus, Bacillus sibtilis, klebsiella pneumonia was also observed. Trichoderma viride and penicillium citrinum was found to be resistant to aqueous extract. To determine the binding interaction, in silico docking analysis was carried out between important phytoconstituents of L. reticulata, reference molecule and macromolecular enzymes that involved in key cellular biosynthetic process. Stigmasterol, a bioactive compound from L. reticulata exhibited significant docking score of -44.669kcal/mol with Dihydrofolate synthase. Similarly, Diosmetin and beta-sitosterol also exhibited remarkable binding affinity (-43.6159kcal/mol, -42.9215kcal/mol) with Dihydrofolate synthase. Our study further opens new avenues for further exploitation of the bioactive compounds from this important medicinal plant for pharmaceutical application.

Keywords

Jivanti, Molecular Docking, Antibacterial Assay, Antifungal Assay

Conclusion

The present study validates that L. reticulata has potent bioactive phytoconstituents like Stigmasterol, Diosmetin, beta-sitosterol which are capable of inhibiting microbial growth. However, detail characterizations of the other phytocompounds are required to explore their mode of action and to evaluate the clinical and biosafety potential.

References

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How to cite this article

Sudipta Kumar Mohanty, Indu B.K., Jesse S. Dondapati and Anuradha M. (2023). In vitro and In Silico Evaluation of Anti-Microbial Potential of Leptadenia reticulata - An Endangered Medicinal Plant. Biological Forum – An International Journal, 15(5): 1699-1704.