In vitro and in vivo Evaluation of Chemical Fungicides against Sclerotium rolfsii causing Collar Rot of Chickpea

Author: Bana Sravani and Ram Chandra

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Abstract

Collar rot of chickpea is well known disease in India with 2-5% of losses every year which may even reach up to 60% under severe conditions. Several chemical fungicides are widely available for controlling this disease and many chemicals have developed resistance against the disease. This study aims to cut down the cost of cultivation occurring due to use of non-effective chemicals and to provide hike in farmer’s income. An experiment was conducted in laboratory and Agriculture Farm, BHU, Varanasi to assess the efficacy of some new fungicides viz. Tebuconazole (Folicur 250EC), Azoxystrobin (Onestar 23%SC), Flusilazole (Cursor 40%EC), Thiophanate Methyl + Kasugamycin (Kasu 44.8% + 2.6%) and Thiophanate Methyl (Roko 70%WP) against Sclerotiumrolfsii. These fungicides were prepared at two different concentrations viz. 100 and 300 ppm to evaluate their toxicity. Irrespective of the concentration, Flusilazole and Tebuconazole significantly inhibited the mycelial growth under in vitro con

Keywords

chemicals, fungicides, collar rot, chickpea, Sclerotium rolfsii

Conclusion

All the tested fungicides were found to be more or less effective against the pathogen at 100 and 300 ppm concentrations under in vitro conditions. Complete inhibition of pathogen was found when treated with Flusilazole and Tebuconazole at all concentrations tested. Thiophanate Methyl + Kasugamycin have also reported about 91.29 and 94.37% at 100 and 300 ppm respectively. When Thiophanate Methyl was tested alone little decline in inhibition rate was noticed resulting in 80.74 at higher concentration. On the other hand, Azoxystrobin was reported to be least effective against pathogen. Same fungicides were tested under field conditions against pathogen. The results obtained were co-related to that of in vitro conditions. Thus, Tebuconazole and Flusilazole treated plants have resulted with less disease severity of about 12% with 100 ppm concentration and below 7.77% with 300 ppm concentration. So, final yield obtained was much higher in these plants with 29% higher yield than control res

References

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How to cite this article

Sravani, B. and Chandra, R. (2021). In vitro and in vivo Evaluation of Chemical Fungicides against Sclerotium rolfsii causing Collar Rot of Chickpea. Biological Forum – An International Journal, 13(2): 10-16.