In-vitro and in-vivo Evaluation of Fungicides Against Collar Rot of Chickpea (Sclerotium rolfsii)

Author: Abhishek Sahu, R.K.S. Tiwari, V.K. Nirmalkar, P.K. Keshary and N.K. Chaure

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Abstract

The efficacy of fungicides was tested at different concentrations of 100ppm, 500ppm, 1000ppm and 2000ppm against Sclerotium rolfsii on PDA by poisoned food technique in-vitro condition and seed treatment with fungicides under pot experiment. It was concluded that fungicides like, Metiram 70%WG, Tricyclazole 75% WP, Propiconazole 25% EC, Azoxystrobin 18.2% + Difenoconazole 11.4% SC, Hexaconazole 5% SC, Iprobenfos 48% EC, Azoxystrobin 11% +Tebuconazole 18.3% SC, Thifluzamide 24% SC, Mancozeb 75% WP, Propineb 70% WP, Tebuconazole 50% + Trifloxystrobin 25% WG, Tebuconazole 38.39% w/w SC, Hexaconazole 4% + Zineb 68% WP and Metiram 55% + Pyraclostribin 5% WG were completely inhibitory at 100ppm concentration showing high potential against Sclerotium rolfsii.While, Kasugamycin 3% SL showed complete inhibition of the pathogen at 500ppm concentration. Fungicide i.e. Validamycin 3% showed partial inhibition of pathogen at 500ppm, 1000ppm and 2000ppm concentrations respectively. Similar results was found in case of seed treatment with fungicides under pot experiment. Fungicides which include phenylpyrroles, anilinopyrimidines, strobilurin analogues etc with effects on respiration, cell membranes components, protein synthesis, signal transduction and cell mitosis

Keywords

Fungicides, Sclerotium rolfsii, collar rot, chickpea

Conclusion

In-vitro study on the efficacy of fungicides against Sclerotium rolfsii indicated that fungicides Metiram 70%WG, Propiconazole 25% EC, Azoxystrobin 18.2% + Difenoconazole 11.4% SC, Hexaconazole 5% SC, Azoxystrobin 11% +Tebuconazole 18.3% SC, Mancozeb 75% WP, Propineb 70% WP, Tebuconazole 50% + Trifloxystrobin 25% WG, Hexaconazole 4% + Zineb 68% WP and Metiram 55% + Pyraclostribin 5% WG were found highly effective and can be used as seed treatment for the control of disease caused by Sclerotium rolfsii. Findings of pot experiment conducted on the efficacy of fungicides as a seed treatment for the control of collar rot of chickpea indicated that fungicides i.e. Hexaconazole 4% + Zineb 68% WP, Tebuconazole 50% + Trifloxystrobin 25% WG, Hexaconazole 5% SC, Tebuconazole 38.39% w/w SC, Thifluzamide 24% SC, Azoxystrobin 11% + Tebuconazole 18.3% SC, Propiconazole 25% EC were highly effective in controlling pre emergence and post emergence mortality caused by Sclerotium rolfsii causal agent of collar rot of chickpea

References

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How to cite this article

Abhishek Sahu, R.K.S. Tiwari, V.K. Nirmalkar, P.K. Keshary and N.K. Chaure (2024). In-vitro and in-vivo Evaluation of Fungicides Against Collar Rot of Chickpea (Sclerotium rolfsii). Biological Forum – An International Journal, 16(11): 81-86