In vitro Antibacterial Activity and FTIR Analysis of Anisomeles malabarica (L.) Leaves Extract against Mycobacterium marinum Affecting Gold Fish (Carassius auratus)

Author: S. Jeba Sheeba, R. Raja Jeya Sekar, A. Harish and S.M. Vijila

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Abstract

In the present study, Mycobacterium marinum, a bacteria isolated from infected gold fish (Carassius auratus) was tested for its antibacterial potency against Anisomeles malabarica leaf – methanol extracts. Disc diffusion plates on agar were used to measure the antibacterial activity. Different concentrations (0.25l, 0.5l, 0.75l and 1.0 l) of A. malabarica leaf-methanol extract was tested against M. marinum. The A. malabarica leaf - methanol extract was exposed a consequence level of inhibition against M. marinum. Maximum zone was measured after 24 hours at 37°C. The result of the present study revealed that the fish pathogen M. marinum was exhibited maximum zone of clearance (18 mm) at 1.0 l of concentration, where as the minimum zone of clearance (8 mm) was recorded at 0.25l of concentration. Fourier Transform Infra-Red Spectroscopy (FTIR) of A. malabarica leaf – methanol extract revealed the presence of alkenes, nitrogen, aliphatic, oxygen and hydroxyl groups. The Phytochemical analysis of A. malabarica leaf – methanol extract showed the presence of tannins, flavonoids, carbohydrates, phenols, proteins, steroids, saponins and coumarins. As per the result of the present study, it can be concluded that qualitative phytochemicals were identified in A. malabarica leaf - methanol extract was effective against M. marinum, a microbial pathogen that has infected gold fish (C. auratus). To verify these results, in vitro and in vivo experiments are required.

Keywords

M. marinum, A. malabarica, phytochemicals, antibacterial activity, FTIR analysis

Conclusion

The medicinal plants continue to be important therapeutic agents in both conventional and modern healthcare systems. The plant derived extracts are utilized to treat a variety of fish ailments in order to promote natural behavior and immunity as well as to prevent the transmission of infection. A. malabarica is a remarkable medicinal herb with a wide range of therapeutic characteristics, according to phytochemical, TLC and FTIR analysis of its leaves. In this study, M. marinum, a bacterium isolated from infected goldfish (C. auratus) was resistant to A. malabarica leaf – methanol extract, which shown promising antibacterial activity.

References

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How to cite this article

S. Jeba Sheeba, R. Raja Jeya Sekar, A. Harish and S.M. Vijila (2023). In Vitro Antibacterial Activity and FTIR Analysis of Anisomeles malabarica (L.) Leaves Extract against Mycobacterium marinum Affecting Gold Fish (Carassius auratus). Biological Forum – An International Journal, 15(5): 251-253.