In vitro Antioxidant Activity of Aqueous and Alcoholic Extracts of Andrographis paniculata Whole Plant Powder by ABTS free Radical Scavenging Assay

Author: Hariharan T. and Vasan P.

Journal Name:

Download PDF

Abstract

The present investigation aimed to evaluate the antioxidant activities of whole plant extracts of Andrographis paniculata (APWP) by an in vitro model ABTS free radical scavenging assay. Gallic acid, at a concentration of 0.5, 1.0, 1.5, 2.0 and 2.5 µg/ml was used as a reference inhibitor in the ABTS assay, which showed 15.88, 32.25, 45.62, 61.91 and 72.39 per cent inhibition of ABTS free radicals, respectively, with IC50 value of 1.5 µg/ml (1.33 - 1.70 µg/ml). Both ethanolic and aqueous extracts of APWP at a concentration of 5, 10, 25, 50 and 100 µg/ml showed 2.46, 5.36, 12.64, 24.52 and 41.85 per cent inhibition and 3.68, 6.51, 17.54, 33.27, and 57.30 per cent inhibition of ABTS free radicals, respectively. The IC50 value of the ethanolic and aqueous extract samples in the ABTS radical scavenging assay was 150.2 µg/ml (105.72 - 261.30 µg/ml) and 87.75 µg/ml (67.87 - 117.62 µg/ml), respectively. So, the in vitro studies clearly showed that the whole plant extracts of A. paniculata have a significant antioxidant activity and the aqueous extract was found to be more effective in the hydroxyl radical scavenging activity with a better IC50 value than the ethanolic extract of APWP. It could therefore be concluded that the free radical scavenging activity of the whole plant extract of Andrographis paniculata might be responsible for the therapeutic properties

Keywords

Andrographis paniculata, antioxidant activity, ABTS, free radical scavenging assay

Conclusion

The results of the present study clearly showed that both ethanolic and aqueous extracts of APWP have potent anti-oxidant properties, which can be used as natural antioxidants for treatment of wide variety of disease conditions ranging from pyrexia to COVID-19 not only in humans, but also in animals.

References

-

How to cite this article

Hariharan T. and Vasan P. (2024). In vitro Antioxidant Activity of Aqueous and Alcoholic Extracts of Andrographis paniculata Whole Plant Powder by ABTS free Radical Scavenging Assay. Biological Forum – An International Journal, 16(11): 158-162