In vitro Antioxidant, Antibacterial activity and Phytochemical Characterization of Senecio nudicaulis Buch. -Ham. er D. Don leaves extracts

Author: Pritee Pant, Kapil Khulbe and Charu C. Pant

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Abstract

The antioxidant and antibacterial activities of Senecio nudicaulis (Asteraceae) are elucidated in the current paper, along with chemical characterization using its methanol and hexane extracts. The highest levels of phenolics (78.26 ±0.86 mg GAE/g dry weight) and flavonoids (32.10 ±0.24mg QE/g dry weight) were detected in the methanol extract, which also displayed remarkable antioxidant potential in DPPH (IC50-30.74 ±1.93 µg/mL), FRAP (120.11 ± 2.21mg AAE/g dry weight), and ABTS (IC50 - 28.61±0.25 µg/mL) assays. Methanol extract showed the highest level of inhibition (13.66 mm) against A. tumefaciens, followed by hexane extract (12.5 mm) against P. aeruginosa and A. tumefaciens. Methanol extract against A. tumefaciens and hexane extract against P. aeruginosa both had substantial MIC values (12.5 mg/ml). GC-MS analysis revealed that 6-Methyl-3,5-heptadiene-2-one (27.9%), 2,5-dihydroxy benzene acetic acid (15.63%), and hexahydroindanin (11.37%) were dominated in methanol extract, whereas phytyl palmitate (9.98%), 9,19-Cyclo-27-norlanostan-25-one, 3-(acetyloxy)-24-methyl-(3.β.,24R) (5.77%), stearic acid (8.08%), dihomo-γ-linolenic acid (9.40%), and phytol (8.21%)were found to be the major constituents in hexane extract. While non-polar extract did show significant antibacterial activity, polar extract exhibited strong antioxidant potential. Results clearly demonstrated antioxidant and antibacterial activities due to the presence of bioactive phytochemicals. The present study makes a foundation for development of new and effective antibiotic and antioxidant drugs.

Keywords

Senecio nudicaulis, GC-MS, Phytochemicals, Antioxidant, Antibacterial, Medicinal plants

Conclusion

The current paper is the first to detail the chemical makeup and antioxidant and antibacterial effects of S. nudicaulis methanol and hexane extracts using in-vitro experiments. The present study reports the presence of many medicinally valuable and industrially relevant compounds that have previously been linked to biologically active functions. Tested plant extracts showed antioxidant activity in the DPPH, FRAP, and ABTS assays. Furthermore, bacterial strains were also found to be susceptible to plant extracts.

References

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How to cite this article

Pritee Pant, Kapil Khulbe and Charu C. Pant (2023). In vitro Antioxidant, Antibacterial activity and Phytochemical Characterization of Senecio nudicaulis Buch. -Ham. er D. Don leaves extracts. Biological Forum – An International Journal, 15(5): 205-215.