In Vitro Evaluation of Synergistic Anti-bacterial Effect of Raw Honey and Silk Cocoons Extract

Author: Deepali Sambyal, Naresh Thakur, Nandini Rai, Neelam Saini, Shivani Rana, Sofia and Meenakshi Sharma

Download PDF

Abstract

The study's objective is to ascertain the antibacterial activity of sericin and honey and their synergistic effect when used in combination. One of the largest challenges to world health, food security, and development progress is antibiotic resistance. It has been demonstrated that honey and sericin exhibit anti- bacterial effect on antibiotic-resistance strains. Considering the significant anti-microbial activity of honey and sericin, we have tested the synergistic anti-bacterial effect of raw honey and cocoons extract. Different concentrations of honey and sericin, the mixture was prepared to determine the anti-bacterial synergism. These samples were tested against bacterial strains of E. coli, S. aureus, P. aeruginosa and Klebsiella sp. The Kirby- Bauer disk diffusion technique was used for susceptibility testing. Over the MHA surface, the test organism was equally planted Microbial growth was assessed on MHA plates after 24 hours of incubation period. Honey and sericin inhibited the growth of all the test organisms. However, the endeavor is confronted by several challenges, standardization of raw materials is essential to account for variations, and method development must consider bacterial strains, optimal component ratio, and potential interactions. Overcoming has been done by standardization of the materials. When honey mixed with sericin, mixture showed higher antibacterial activity. Study showed that honey and sericin may be effective antibacterial future agents against resistant bacterial strains when used as combination. Sericin and honey have been found to provide health benefits for several ailments, such as microbial infections and wound healing.

Keywords

Antibacterial activity, Silkworm, cocoons, Sericin

Conclusion

Finally, it was concluded that mixture of sericin and honey have efficient ability to inhibit the growth of bacterial strains. Therefore, honey and sericin could be used as a part of combination treatment for resistance bacterial infection.

References

-

How to cite this article

Deepali Sambyal, Naresh Thakur, Nandini Rai, Neelam Saini, Shivani Rana, Sofia and Meenakshi Sharma (2023). In-Vitro Evaluation of Synergistic Anti-bacterial Effect of Raw Honey and Silk Cocoons Extract. Biological Forum – An International Journal, 15(10): 1445-1450.