Indexing

In Vitro Evaluation of the Anti-inflammatory and Antioxidant Properties of Mesua ferrea Linn. Stem Bark Extract

Author: Jyothsna K., Manjunatha H. and Ravi Kumar S.

PDF Download PDF

Abstract

Plants rich in antioxidants are also a potential source of and exhibit anti-inflammatory effects paving the path for the development of new drugs with minimal/no side effects. Hence, in this study, the antioxidant and anti-inflammatory properties of the ethanolic extract of Mesua ferrea Linn. stem bark were evaluated using various in vitro methods. The qualitative and quantitative analyses were conducted to determine the presence of secondary metabolites. The extract exhibited significant antioxidant activity, as demonstrated by its ability to scavenge 2,2-Diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, hydroxyl radicals, and metal chelating ability. The extract's anti-inflammatory activity was also assessed by measuring the inhibition of pro-inflammatory cytokines, NO, PGE2 production, HRBC membrane stabilization, protein denaturation, inhibitory effects on proteinase, lipoxygenase and cyclooxygenase. The results showed that the ethanolic extract possessed substantial antioxidant and anti-inflammatory properties, making it a promising source for the development of novel drugs with minimal side effects.

Keywords

Mesua Ferrea Linn., Antioxidant, Anti-inflammatory, Macrophages, cytokines

Conclusion

The current study demonstrates that the ethanol extract of MF stem bark exhibits significant antioxidant and anti-inflammatory effects. The presence of alkaloids, flavonoids, and other phenolic compounds in the extract contribute to these observed activities, as they exhibit dose-dependent scavenging of free radicals. Moreover, our findings indicate that the extract effectively inhibits the production of TNF-α, IL-1β, IL-6, NO, and PGE2 in LPS-stimulated THP-1 cells. Further investigations are required to identify the active constituents of the extract and elucidate their mechanisms of action.

References

-

How to cite this article

Jyothsna K., Manjunatha H. and Ravi Kumar S. (2023). In Vitro Evaluation of the Anti-inflammatory and Antioxidant Properties of Mesua ferrea Linn. Stem Bark Extract. Biological Forum – An International Journal, 15(5): 308-318.