In vitro Propagation and Genetic Assessment by using Molecular Markers in a New Potato (Solanum tuberosum L.) cv. Kufri Pushkar

Author: Tejinder Singh, V.P.S. Panghal, Subhash Kajla, Bichhinna Maitri Rout, Anil Poonia and Love Sapra

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Abstract

The aim of our experiment was to determine the optimal types and concentrations of plant growth regulators used during different stages of micropropagation and assess the genetic fidelity of tissue culture raised plants of a cv. Kufri Pushkar by using reliable markers. The data of shoot bud initiation indicated that combination of 0.25 mg l-1 BAP and 0.5 mg l-1. Kinetin leads to highest number of shoots per explant (3.6±0.07) in (3.3±0.08) days. The maximum number of in vitro shoots per shoot let (4.4±0.15) were recorded when auxins were used in combination with cytokinin (MS medium + 0.25 mg/l BAP + 0.01 mg/l IAA) for shoot proliferation. In vitro root initiation was observed in (2.3±0.10) days on MS medium fortified with 1.5 mg l-1 NAA. The maximum number of in vitro roots per shoots (14.0±0.37) were observed when MS media fortified with 2.0 mg l-1 IBA. In vitro raised plants were assessed for genetic fidelity by using molecular markers. DNA banding patterns of all tissue culture raised plants and mother plants were monomorphic showing true to type planting material. This protocol for tissue culture propagation along with testing its genetic fidelity could be useful for successful application of gene transfer technique.

Keywords

Shoot proliferation, micropropagation, Solanum tuberosum L., genetic markers

Conclusion

An efficient micropropagation via shoot tips as initial explants along with its genetic fidelity was described for potato cv. KufriPushkar. The studies could also be expanded for the successful application of gene transfer technique and better conservation of germplasm. However, this work will contribute to the global demand for the production of high-quality virus-free potato planting material, to enhance the yield and productivity of the crop.

References

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How to cite this article

Tejinder Singh, V.P.S. Panghal, Subhash Kajla, Bichhinna Maitri Rout, Anil Poonia and Love Sapra (2023). In vitro Propagation and Genetic Assessment by using Molecular Markers in a New Potato (Solanum tuberosum L.) cv. Kufri Pushkar. Biological Forum – An International Journal, 15(10): 59-67.