In vitro Regeneration of Musa Spp. Plantlet CV. Grand Naine by Plant Tissue Culture Technique

Author: Rahul R. Rodge, Anis Mirza, Harjinder Kaur, Lakhesing Girase and Khan Jabroot J.

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Abstract

Banana (Musa spp.) is one of the most consumable fruits and cultivated around the globe. It was majorly propagated through tissue culture technique. It contains high nutritional value as well as the high demand of the market. There are many challenges in banana production long gestation durations, low yields, excessive flood irrigation resulting to high mortality rates, and trouble propagating disease-free uniform suckers were problems that conventional banana farming techniques started to cause for growers. These difficulties may result from several things, including a scarcity of disease-free plants and planting materials or farmers who have not been exposed to new technology we contribute way to produce good quality Banana Tissue culture plant using with PGR and AC. To enhance the tissue culture technique for production of cv. Grand Naine plantlets we use 6-Benzylaminopurinefor shoot proliferation and Indole butyric acid and Activated charcoal for more root proliferation investigate the effect of different cytokinin concentrations, such as BAP (6-benzyl amino purine), on a shoot, leaf, and multiplication, as well as different auxin concentrations, such as IBA (Indole 3-butyric acid) + (AC) Activated charcoal, on root proliferation, to develop a protocol for in vitro plant regeneration from shoot tip explants of the Grand Naine variety of Banana. Different concentrations of BAP (0.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 mg/l) and IBA (0.5, 1.0, 1.5 mg/l) combination with AC (1.0, 1.5, 2.0 gm/l). The highest number of the shoot after 55 days was observed (6.16) MS medium supplemented with (4.0 mg/1 BAP) the highest length of shoot (5.76) cm that was recorded in MS medium supplemented with (4.0 mg/1 BAP) and highest number of leaves (4.5) found in MS medium supplemented with (4.0 mg/1 BAP) highest length of leaves 4.13 recorded in MS medium supplemented with (3.0 mg/l BAP) and highest number of Root recorded (5.0) in MS supplemented (2.0 gm/l AC+1.5 mg/l IBA) highest length of Root 8.25 recorded in MS supplemented (2.0 gm/l AC+1.5 mg/l IBA) respectively root proliferation recorded in 95 days. In the present study, we found (4.0 mg/l BAP) is best for a shoot, leaf proliferation, and multiplication, and AC + IBA is best for Root.

Keywords

BAP cytokinin, IBA auxin, Activated charcoal, in vitro shoot, leaf, Root proliferation

Conclusion

Application BAP Growth hormone supplemented on MS culture medium at 4.0 mg/l and 5.0 mg/l performs better for new plantlet regeneration capabilities via in vitro shoot-tip culture. BAP concentration of 4.0 mg/l was shown to be the optimum concentration for shoot proliferation, shoot elongation, and leaf proliferation in the Banana cv. Grand Naine variety. For Root Proliferation and Root Elongation, Activated Charcoal (AC) is ideal for the combination with IBA growth regulators This combination gives the highest number of roots (2.0 g/l AC + 1.5 mg/l IBA) and the longest root length (2.0 g/l AC + 1.5 mg/l IBA) suitable for rooting proliferation alone IBA is not that much effective if we use Activated charcoal combine with IBA is best for Root proliferation early growth in tissue culture Banana on commercial production.

References

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How to cite this article

Rahul R. Rodge, Anis Mirza, Harjinder Kaur, Lakhesing Girase and Khan Jabroot J. (2023). In vitro Regeneration of Musa Spp. Plantlet CV. Grand Naine by Plant Tissue Culture Technique. Biological Forum – An International Journal, 15(4): 533-538.