Induction of Antioxidants by PGPR to Mitigate Stress by Pathogen F. oxysporum in Chickpea Variety KWR-108 under Drought

Author: Swapnil B. Matikhaye*, Bethala Kumeera, D.P. Gogle, Suchit A. John and P.W. Ramteke

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Abstract

The present study was conducted during 2019-2020 and 2020-2021 at MBGE Department, RTMNU, Nagpur, Maharashtra to assess the effects of PGPR with biocontrol activity on antioxidant parameters under drought conditions in situ (field) studies. Three potential PGPRs namely Pseudomonas spp, E. cloacae and P. chlororaphis were used in the present study. The pooled data of two experiment results of antioxidant enzymes recorded T7 (A+B) PGPR treatment with consortium of Pseudomonas spp. and P. chlororaphis ranged highest in proline with 5.37 (µg/g). In SOD highest recorded with 8.13 (U/mg) in T7 (A+B), PAL ranged highest between 1.76 (mg/L), catalase with 15.56 (µg/g) in T7 treatment according to the pooled data. However the lipid peroxidation and H2O2 content indicated as toxic levels caused by over production of ROS during water deficit stress. This could overcome by decreasing levels of MDA through PGPR T7 treatment with 8.67 (Mm-1 cm-1), while increased in T3 with 12.39 (Mm-1 cm-1) thus

Keywords

Chickpea, Fusarium oxysporum, PGPR, drought, Biochemical.

Conclusion

From the results, it is concluded that T7 (A+B) is the best combination treatment comprising of Pseudomonas spp. and Pseudomonas chlororaphis in control of Fusarium oxysporum and mitigating drought stress in KWR-108 variety of chickpea in terms of antioxidant parameters such as proline, superoxide dismutase, catalase enzymes increased inorder to overcome negative effects of ROS, whereas lipid peroxidation and hydrogen peroxide decreased due to reported PGPR in this study by decreasing the toxic levels that occur during drought conditions. Therefore PGPR would be suitable reason to conquer reported pathogen even under water deficit conditions.

References

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How to cite this article

Swapnil B. Matikhaye, Bethala Kumeera, D.P. Gogle, Suchit A. John and P.W. Ramteke (2022). Induction of Antioxidants by PGPR to Mitigate Stress by Pathogen F. oxysporum in Chickpea Variety KWR-108 under Drought. Biological Forum – An International Journ