The phenol exudation from explants of woody plant species increases the rate of oxidative browning that causes death of the majority of cultured explants. A study was carried out to identify the best antioxidant for in vitro culture establishment and to minimize the phenol exudation in explants of clonal apple rootstocks MM106 and MM111. The experiment was carried out at Plant Tissue Culture laboratory of School of Biotechnology and Tissue Culture laboratory of Advanced Centre for Horticulture Research, Udheywalla of SKUAST-Jammu during the year 2018. Results indicated that MS + 10 g PVP (AM4) resulted in highest culture establishment in MM111 and MM106 clonal apple rootstocks. The shoot tip and nodal segment explants of MM111 rootstock recorded highest culture establishment i.e. 47.88 per cent and 35.33 per cent, respectively, whereas 32.22 per cent and 27.27 per cent culture establishment was obtained in shoot tip and nodal segment, respectively in MM106 rootstock.

Antioxidants, in vitro, culture establishment, MM106 and MM111.

The phenol exudation from explants of woody plant species increases the rate of oxidative browning that causes death of the majority of cultured explants. A study was carried out to identify the best antioxidant for in vitro culture establishment and to minimize the phenol exudation in explants of clonal apple rootstocks MM106 and MM111. The experiment was carried out at Plant Tissue Culture laboratory of School of Biotechnology and Tissue Culture laboratory of Advanced Centre for Horticulture Research, Udheywalla of SKUAST-Jammu during the year 2018. Results indicated that MS + 10 g PVP (AM4) resulted in highest culture establishment in MM111 and MM106 clonal apple rootstocks. The shoot tip and nodal segment explants of MM111 rootstock recorded highest culture establishment i.e. 47.88 per cent and 35.33 per cent, respectively, whereas 32.22 per cent and 27.27 per cent culture establishment was obtained in shoot tip and nodal segment, respectively in MM106 rootstock.

Lal, M., Jamwal, M., Bakshi, P., Jasrotia, A. Sharma, N., Sharma, M., Singh, P., Sharma, S. and Kumar, S. (2021). Influence of Antioxidants on in vitro Culture Establishment of Clonal Apple Rootstocks. Biological Forum – An International Journal, 13(2):