Influence of Supplementary Carbon and Nitrogen Sources on Pyrene Degradation by Aspergillus lacticoffeatus Isolated from Crude Oil-contaminated Soil

Author: Veeramani Veerasamy, Gowri Manogari Boovaragamoorthy and Thamaraiselvi Kaliannan

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Abstract

Polycyclic aromatic hydrocarbon is the most prevalent environmental pollutant. In this study, pyrene degrading fungi, Aspergillus lacticoffeatus, was isolated from crude oil-contaminated soil from Oil and Natural Gas Corporation (ONGC) Karaikal. A. lacticoffeatus is optimized for pyrene degradation in the medium amended with different nitrogen and carbon sources. Among the carbon sources, it was found that pyrene degradation was significant with sucrose compared to glucose, fructose, maltose, and starch. The ammonium chloride nitrogen source provided better degradation than ammonium sulfate, ammonium nitrate, calcium nitrate, and sodium nitrate. Maximum pyrene removal was obtained in the combination of sucrose and ammonium chloride (84.91%), and physical parameters like pH (6.00) and temperature (35°C) provided a better degradation in a period of 30 days. Overall, the study identified glucose and sucrose as suitable carbon sources and ammonium chloride as a suitable nitrogen source for pyrene degradation.

Keywords

Pyrene degradation, Polycyclic aromatic hydrocarbon, Carbon sources, Nitrogen sources, Aspergillus lacticoffeatus

Conclusion

This study dealt with isolating a novel strain of Aspergillus lacticoffeatus isolated from a crude oil-contaminated site for effective pyrene degradation. Results showed significant variance in the growth and pyrene removal pattern for Aspergillus lacticoffeatus for different carbon and nitrogen sources. High mycelial biomass and pyrene degradation in glucose and sucrose-containing medium was seen in the presence of ammonium chloride as a nitrogen source. Among glucose and sucrose, sucrose was seen to be more effective for removing pyrene with ammonium chloride by Aspergillus lacticoffeatus. Hence this fungal strain showed promising properties for the effective degradation of pyrene.

References

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How to cite this article

Veeramani Veerasamy, Gowri Manogari Boovaragamoorthy and Thamaraiselvi Kaliannan (2023). Influence of Supplementary Carbon and Nitrogen Sources on Pyrene Degradation by Aspergillus lacticoffeatus Isolated from Crude Oil-contaminated Soil. Biological Forum – An International Journal, 15(5): 93-98.