To fully understand the microbial potential of bioagents on the banana's phylloplane with the goal to use the right phylloplane microflora to manage banana diseases efficiently. The experiment featured a completely randomized design and had been carried out in a laboratory environment. To do this, the phylloplane microflora associated with healthy and diseased leaves were studied using a modified leaf washing strategy. The dual culture approach was used to evaluate the isolated phylloplane microflora's ability to combat banana pathogens in vitro. Two bacteria, Bacillus sp. and Pseudomonas sp., and four fungus, Colletotrichum sp., Trichoderma sp., Aspergillus niger, and Penicillium sp., were recovered from the phylloplane microflora of both healthy and diseased banana leaves. By using the dual culture method, five phylloplane microflora were tested for their antagonistic effect against Colletotrichum sp. The results indicated that the treatment of Trichoderma sp. (9.75 mm) resulted in the significantly least growth of the pathogen, as the Trichoderma sp. overgrew the Colletotrichum sp. colony, preventing further growth and sporulation. Trichoderma sp. treatment (88.95%) and Aspergillus niger treatment (81.30%) showed the greatest growth inhibition of Colletotrichum sp. Incredibly varied and diverse fungal species were found on leaf surfaces which can be used as effective as a bio control for effective management of plant diseases. So from this study it can be stated that Trichoderma sp. found from banana phylloplane were found effective bio agent against Colletotrichum sp

Phylloplane Microflora, Banana, Trichoderma sp., Colletotrichum sp

Studies of the banana's phylloplane microflora revealed that there were more fungi isolated than bacteria. When comparing the phylloplane microflora of diseased and healthy banana leaves, the diseased ones had a significantly higher population than the other one. Trichoderma sp. demonstrated a high level of antagonistic activity in the phylloplane microflora's antagonistic activity against the banana pathogen, directly inhibiting the growth of the pathogen. Therefore, it is possible to use Trichoderma sp. as antagonists against banana foliar pathogens. Further these effective phylloplane microflora can be mass multiplied and can be assessed at field level

S.S. Kansara, M.I. Patel, V.P. Prajapati, S.K. Chawada and Denny Tandel (2025). Investigation of Phylloplane Microflora of Banana. Biological Forum – An International Journal, 17(1): 87-89