Isolation and Efficacy Evaluation of Forest Soil Microflora (FSM) Against Sclerotium rolfsii (Sacc.) causing Stem Rot of Groundnut under in vitro Conditions

Author: Archana N., Srilatha P., Vidysagar B., Pavani Y. and Avanija M.

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Abstract

Major threat in chemical control of plant diseases is the fungicide resistance, environmental pollution and health hazards. Therefore, an ecofriendly approach such as biological control is necessary. Biological control play a powerful role for the treatment of bacterial and fungal plant diseases. Bacteria with a variety of mechanisms involved in limiting the spread of plant diseases are some of the most extensively studied biological control agents. A total of 42 bacteria and 36 fungi were isolated from the various regions of Katkur Reserve Forest and Tirumala kunta Reserve Forest under Bhadradri Kothagudem district following dilution method. The conventional dual culture technique was used to further confirm the antagonistic activity of these forest soil isolates. On the basis of the outcomes, the potential isolates were chosen. The potential isolates FSF10, FSB2, FSB4, FSB16 and FSF30 inhibited fungal growth by 76.44%, 61.12%, 63.78%, 60.76 % and 64.79% respectively, in a dual culture test. The study suggests that the FSF10 (Trichoderma erinaceum) has highest potential to be used as biocontrol agent against Sclerotium rolfsii causing groundnut stem rot.

Keywords

Antagonistics, Forest soil microbes, Groundnut stem rot, Trichoderma erinaceum, Bacillus spp

Conclusion

In this experiment, 42 bacterial and 36 fungal cultures were isolated and screened against the groundnut stem rot causing pathogen Sclerotium rolfsii. through their biocontrol mechanisms. Among them 18 forest soil bacterial isolates and 13 fungal isolates were shown more than 40 % of inhibition of radial growth of pathogen. The isolates FSF10, FSB2, FSB4, FSB16, FSB30 were the potential isolates showing highest percent of inhibition with FSF10 showing maximum percent inhibition. Based on the morphological and molecular characterization the FSF10 isolate is identified as Trichoderma erinaceaum. So these isolates can be further screened at field level and can be used as biocontrol agents against groundnut stem rot disease as an ecofriendly control measure of disease.

References

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How to cite this article

Archana N., Srilatha P., Vidysagar B., Pavani Y. and Avanija M. (2023). Isolation and Efficacy Evaluation of Forest Soil Microflora (FSM) Against Sclerotium rolfsii (Sacc.) causing Stem Rot of Groundnut under in vitro Conditions. Biological Forum – An International Journal, 15(10): 1390-1394.