Isolation and Molecular Identification of Bacterial Species from Vindhyachal forest Madhya Pradesh for Biological Control of Early Blight

Author: Omshri Singh and Deepak Bharti

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Abstract

The rhizosphere, a narrow region of soil in direct contact with root secretions and significantly influenced by soil microorganisms, was the focus of a recent study. This study examined the microflora of the Vindhyachal forest in Madhya Pradesh, with an emphasis on the isolation and molecular identification of bacterial species from the forest’s remote areas. These microorganisms may have potential applications in the biological control of Early Blight, a disease commonly affecting potato and tomato crops. The study faced difficulties in the isolation and molecular identification of bacterial species from remote areas of the Vindhyachal forest, which required precise collection and handling of soil and plant root samples. By exploring the microbial diversity in these areas, the aim is to identify potential biocontrol agents that could provide an environmentally friendly and sustainable solution to managing Early Blight. Soil samples from the rhizosphere were collected using a standard protocol. Plant root materials, known as rhizoplanes, were also collected during October and November from remote areas of the Vindhyachal forest in Madhya Pradesh. The samples were serially diluted and streaked over Nutrient Agar media plates for bacterial isolation. The cultured bacteria were further analyzed for identification through molecular analysis using PCR and sequencing of the 16S region. This groundbreaking study illuminates the intricate world of microbiology, revealing the pivotal role of various bacterial strains in fostering plant health and safeguarding them from harmful pathogens. The bacteria under investigation include Pseudomonas fluorescens, Priestia megaterium, Bacillus subtilis, and a strain of Bacillus licheniformis. This study highlights the crucial role of certain bacteria in promoting plant health and protecting potatoes from pathogens, including Early Blight. These bacteria could lead to more sustainable farming practices and enhance our understanding of plant-microbe interactions.

Keywords

Biological Control, Early Bligh, Molecular identification, 16S RNA, Vindhyachal forest

Conclusion

In present study discovered that the bacterial isolates from the rhizoplane of wild plants in a natural forest ecosystem belonged to four distinct genera: Pseudomonas, Bacillus, Priestia, and Rhizobium. These genera are renowned for their plant growth-promoting and biocontrol properties, including the production of siderophores, antibiotics, hydrolytic enzymes, bioactive compounds, and nitrogen fixation. Our research has shed light on the diversity and potential of these rhizoplane bacteria, which could have significant implications for plant health and productivity, as well as soil quality and sustainability.

References

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How to cite this article

Omshri Singh and Deepak Bharti (2023). Isolation and Molecular Identification of Bacterial Species from Vindhyachal forest Madhya Pradesh for Biological Control of Early Blight. Biological Forum – An International Journal, 15(4): 977-981.