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Isolation and Screening of Amylolytic Yeast from various Natural Sources

Author: A. Veena and B.S. Anuradha

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Abstract

The objective of this research is to get the isolates of yeast which have the potential to produce amylase. Amylase enzymes are the examples of the types of enzymes that play an important role in biotechnology and industry. Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and safer for the environment. Amylolytic yeast can be isolated from flower substrates as it contains sugar for the very limited condition of yeast growth. Amylase is one of most important group of enzymes. Amylolytic yeast strains were isolated from different natural sources. These are of ubiquitous occurrence and hold the maximum marked share of enzyme sales. Which hydrolyze the starch it is observed by the zone of inhibition by the adding of iodine. Starch converts to product as maltose and glucose molecules. Amylase having yeasts were isolated on the YEPD agar media. These isolated colonies were centrifuged at 8000 rpm for 15min by adding methanol as a substrate. The crud was extracted and all isolates were screened on YEPS (Yeast extract Peptone Starch) media, for the amylase activity crude extract possess zone of inhibition by the lysis of starch. The maximum amylase activity having isolate was subjected to 18s rRNA gene sequencing.

Keywords

Amylase enzyme, amylolytic Yeast, Starch, YEPD media and YEPS media

Conclusion

In conclusion total 6 yeast strains were isolated from the different sources like toddy, wine, apple, soil, custard apple and potato. Isolated yeast strains were screened based on clear zone of inhibition by the starch hydrolysis. Based on amylolytic activity among 6 yeasts one was selected and subjected to 18s rRNA gene sequencing the selected isolate can be characterized further for various useful industrial purposes.

References

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How to cite this article

A. Veena and B.S. Anuradha (2023). Isolation and Screening of Amylolytic Yeast from various Natural Sources. Biological Forum – An International Journal, 15(5): 450-454.