Isolation, Characterization, and Antagonistic Activity of Multifaceted Plant Growth-Promoting Bacteria, Including Metaltolerant Lysinibacillus macroides, From Vegetable-Cultivated Soils in Namakkal District, Tamil Nadu, India

Author: R.C. Senthamil Selvi and N. Thangaraj

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Abstract

The study isolates and characterizes multifaceted bacteria, like Lysinibacillus macrolides, with metal tolerance and pathogen control, showcasing their potential in sustainable agriculture and environmental management. The aim of this work is to isolate and characterize multifaceted microorganisms, particularly Lysinibacillus macrolides, for sustainable agriculture and environmental management. Soil samples from Namakkal were used to isolate rhizospheric bacteria via serial dilution plating. Isolates were evaluated for multiple plant growth-promoting traits, metal resistance, and antagonistic activity against phytopathogenic fungi. DNA sequencing was employed for isolate identification. The study identified 25 bacterial isolates with multiple plant growth-promoting traits, metal tolerance, and antagonistic activity. Lysinibacillus macroides exhibited high lead and chromium tolerance and effective antifungal activity against Fusarium sp., with an 84% growth inhibition. Based on the results, it can be concluded that the isolates are the best and can be used to improve soil fertility and this PGP bacterium was hold great promise for sustainable agriculture and environmental improvement.

Keywords

PGPF, Siderophore, Metal resistance, Lysinibacillus macroides, Antagonistic activity

Conclusion

PGP bacteria like Lysinibacillus macrolides offer a comprehensive solution for sustainable agriculture and environmental management. Their unique combination of PGP traits, metal tolerance, and pathogen control reduces reliance on chemicals, enhances crop health, and improves soil fertility. This study broadens our understanding of diverse PGP isolates with specific traits, including nitrogen fixation and enzyme production. Notably, the discovery of lead tolerance in Lysinibacillus macrolides has implications for polluted soil remediation. Additionally, their potent antifungal activity against Fusarium sp. underscores their role as a natural crop defense mechanism, reducing the need for synthetic pesticides. This PGP bacterium was hold great promise for sustainable agriculture and environmental improvement.

References

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How to cite this article

R.C. Senthamil Selvi and N. Thangaraj (2023). Isolation, Characterization, and Antagonistic Activity of Multifaceted Plant Growth-Promoting Bacteria, Including Metaltolerant Lysinibacillus macroides, From Vegetable-Cultivated Soils in Namakkal District, Tamil Nadu, India. Biological Forum – An International Journal, 15(10): 938-944.