Isolation, Characterization and Identification of Microorganisms from Distillery Effluent Contaminated Soil and Ex- situ Bioremediation of Contaminated Soil

Author: Janmeet Kour*, P. K. Chauhan** and Kanika Dulta**

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Abstract

In the present study the effluent samples were collected from the distillery industry situated at Kasauli, Solan, Himachal Pradesh, India. Then the samples were subjected to heavy metal analysis and isolation of heavy metal resistant bacteria. The study revealed the presence of six heavy metals i.e. Pb, Cd, Zn, Cu, Mg and Cr in the distillery effluent contaminated soil. All the heavy metals were above the permitted limits, i.e. Pb (189.02 mg/kg), Cd (5.59 mg/kg), Zn (590 mg/kg), Cu (1065.27 mg/kg), Mg (409 mg/kg) and Cr (196.20 mg/kg). The four isolates were selected based on heavy metal tolerance and antibiotic resistances. These heavy metal resistant strains were characterized and identified on the basis of 16S rRNA sequencing as Proteus vulgaris, Bacillus subtilis, Micrococcus luteus and Bacillus cereus. These selected isolates were examined for their heavy metal uptake capacity and found that isolates had potential for metal uptake. Ex-situ bioremediation was carried out using micr

Keywords

Distillery effluent, heavy metals, 16S rRNA sequencing, ex-situ bioremediation

Conclusion

Environmental laws have become stringent, discharge of the effluent within the permissible limit is mandatory in the developed and developing countries. The distillery wastewater treatment methods practiced by large-scale holders comprise physicochemical methods requiring a large surface area for the set up of effluent treatment plant and technically trained personnel with efficient management skills. It adds to the cost of the treatment process, making it cost intensive. Therefore, biological treatment methods are considered to be ideal and economical. In the present investigation six heavy metals (Pb, Cd, Zn, Cu, Mg and Cr) were detected in distillery effluent contaminated soil. Isolation and screening was carried out to isolate heavy metal resistant bacteria capable of tolerating high concentrations (1000 mg/l) of metal salts. Four selected strains were characterized and identified on the basis of biochemical tests and 16S rRNA sequencing. The four strains were confirmed as Proteus

References

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How to cite this article

Janmeet Kour, P. K. Chauhan and Kanika Dulta. (2018). Isolation, Characterization and Identification of Microorganisms from Distillery Effluent Contaminated Soil and Ex- situ Bioremediation of Contaminated Soil, Biological Forum – An International Journ