Isolation, Identification and Characterization of Colletotrichum gloeosporioides (Penz. and Sacc.) Associated with Post-harvest Anthracnose of Mango

Author: Rahul L. Joshi, V.B. Virani, H.A. Shekhada, D.H. Tandel, Mansi D. Prajapati and Susheel Singh

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Abstract

The mango (Mangifera indica L.) crop is susceptible to numerous diseases, among them anthracnose, induced by Colletotrichum gloeosporioides (Penz. and Sacc.) emerging as a significant menace to mango production. They potentially causing estimated yield losses of 100% in plantations lacking effective management. Various strains within the Colletotrichum complexes are implicated inmangoanthracnose on a global scale. So, the present investigation focused on the isolation, identification and characterization of the Colletotrichum gloeosporioides (Penz and Sacc.) causing anthracnose disease in mango fruits. Mango fruits exhibiting disease symptoms were collected from various markets in the Navsari district and brought to the laboratory. Cultural and morphological characterization revealed that pathogen produce whitish colonies with smooth, hyaline, and sub-cylindrical spores with rounded ends were obtained from anthracnose-infected fruits. The recorded dimensions of conidia ranged from 3.70 to 11.40 µm in length and 1.30 to 2.22 µm in width. Confirmation of the causal organism was achieved through the inoculation of healthy mango fruits using the cork borer injury method. The inoculated fruits exhibited similar disease symptoms within 7-10 days after inoculation, thereby confirming Koch's postulate. The inoculated fungi were produced similar symptoms and Koch’s postulate was proved by re–isolation of the same fungi. Thus, the causal organism of anthracnose under present investigation confirmed as C. gloeosporioides. This study employed a comprehensive approach, combining isolation, morphological identification and Koch's postulate confirmation to provide a thorough understanding of the anthracnose disease dynamics in mangoes.

Keywords

Mango, C. gloeosporioides, Isolation, Identification, Characterization

Conclusion

In conclusion, this investigation confirmed that anthracnose-infected fruits produced whitish colony, conidia were smooth, hyaline and sub cylindrical with round end are observed. Fungal culture obtained from the respective disease symptoms was inoculated into healthy mango fruit by cork borer injury method. The inoculated fungi were produced similar symptoms and Koch’s postulate was proved by re–isolation of the same fungi. Thus, the causal organism of anthracnose under present investigation confirmed as C. gloeosporioides. The study employed a comprehensive approach, combining isolation, morphological identification and Koch's postulate confirmation to provide a thorough understanding of the anthracnose disease dynamics in mangoes. This information is valuable for devising effective management strategies to mitigate the impact of anthracnose on mango cultivation.

References

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How to cite this article

Rahul L. Joshi, V.B. Virani, H.A. Shekhada, D.H. Tandel, Mansi D. Prajapati and Susheel Singh (2023). Isolation, Identification and Characterization of Colletotrichum gloeosporioides (Penz. and Sacc.) Associated with Post-harvest Anthracnose of Mango. Biological Forum – An International Journal, 15(10): 1643-1646.