Management of Collor Rot of Chickpea caused by sclerotium rolfsii through oil Seed Cakes in vitro
Author: Rakesh Gurjar*, A.R. Wasnekar, Mahesh Kumar Mimrot, Yashowardhan Singh, Pushkar Dev
and Jitendra Gurjar
Journal Name:
Download PDF
Abstract
Chickpea is the world’s second most important food legumes next to common bean. Chickpea is a vital source of plant derived edible protein in many countries. It originated in South West Asia and is now grown in over 50 nations. Chickpeas are cultivated on 95.39 lakh hectares in India, with a yield of 90.75 tonnes and a productivity of 951 kg per hectare. Whereas, the total area and production of chickpea in MP is 35.90 lakh hectare and 45.95 lakh tonnes, respectively, having productivity of 1056 kg/ha (Annual Report DPD, 2017-18). Collar rot is a serious soil-borne disease which has directly afflicted chickpea production. Chickpea collar rot is the most serious and difficult disease to control, resulting in substantial yield losses of 60-70 percent under favourable conditions (Nene, 1985). An in vitro study was conducted to evaluate the efficiency of oil cake against S. rolfsii. Required oil cake Neem seed cake, Groundnut cake, Castor cake, Castor cake, Soybean cake, Cotton cake, Mustard cake, Vermicompost and Farm yard manure through the Poisoned Food Technique, respectively. The Neem seed cake extract at 10 and 20 per cent concentration can be effectively inhibiting the growth of Sclerotium rolfsii under in vitro condition. Chickpea is one of important pulse crop which is infected with collar rot oil seed cakes can be used in IDM practice for management of this disease.
Keywords
Sclerotium rolfsii, oil cake, Management, collor rot, Vermicompost
Conclusion
Collar rot disease caused by Sclerotium rolfsii, is a serious threat to chickpea and its control has acquired very limited success. Present investigation was carried out with a view to ascertain the cultural factors responsible for the growth of the Sclerotium rolfsii and management option to minimize the disease. Oil seed cakes like Neem seed cake extract, Groundnut cake extract and Cotton cake have proved to be highly effective in inhabiting the growth of pathogen in vitro at 72 and 120 hrs.
References
INTRODUCTION
Chickpea (Cicer arietinum L) is an important legume crop that ranks second in global agriculture. It belongs to the family Fabaceae, sub family Papilionaceae. Chickpea is a high-protein crop that also improves soil fertility via biological nitrogen fixation. It is produced on 95.39 lakh hectares in India, with an annual production of 90.75 lakh tonnes and a yield of 951 kg per hectare. S. rolfsii is soil borne pathogen and survives in soil for many years (Allce, 1984). The disease causes damage on root and stem of plant. The pathogen produces sclerotia which overwinter in soil and on plant debris and can survive in a long period causing disease in the following season (Punja, 1985). Various methods for controlling such disease have been investigated including the use of mustard cake, caster cake, neem cake, cotton cake, linseed cake, farm yard manure and soybean cake against the S. rolfsii, causing collar rot of chickpea, stem rot and collar rot of groundnut (Dawar, 2010; Kuldhar and Suryawanshi, 2017; Aravind and Brahmbhatt, 2018; Latha and Rajeswari, 2019). In Madhya Pradesh, however, only limited work has been done in the treatment of chickpea collar rot (Sclerotium rolfsii) using organic amendments. The focus of this research is to find the most efficient organic amendments for treating the chickpea collar rot disease.
METHOD AND MATERIAL
A study was conducted to check the efficacy of oil cake against S. rolfsii under in vitro conditions. The pathogen was isolated from infected gram seedlings by hyphal tip method of fungal isolation. Identification of Sclerotium rolfsii were done by morphological characters formed white mat of hyaline mycelium with formation of initially white sclerotia which later turned into brown hard structure. Sclerotia were black, varied from spherical to irregular in shape and measured 80 to 85 µm in diameter. Pycnidial production was not observed in culture plates. Required oil cake Neem seed cake, Groundnut cake, Castor cake, Castor cake, Soybean cake, Cotton cake, Mustard cake, Vermicompost and Farm yard manure (through the Poisoned Food Technique), respectively were obtained from Microbes Research and Production Canter, JNKVV Jabalpur (M.P.). The experiment was conducted during 2019-20, with eight treatments and one untreated control. All the six tested oil cake extracts, Vermicompost and Farm yard manure significantly inhibited the growth of S. rolfsii under in-vitro conditions. However, inhibition in growth of S. rolfsii varied from treatment to treatment. The observation on collar rot of chickpea was recorded at 72 hours and 120 hours by the using of Poison food technique.
Thirty gram well ground powder of each cake was suspended in 150 ml sterile distilled water in flask and left for 25 days. The flasks were shaken for thorough mixing and dissolution of the content. After 25 days the flaks were thoroughly shaken and content were filtered through double layered muslin cloth and autoclaved for 20 minutes. The autoclaved extracts were individually added in previously sterilized melted and cooled potato dextrose agar medium as per required concentration at the time of pouring in Petri plates and mixed thoroughly. All the plates were incubated at 28±1˚C after placing the five mm disc of actively growing seven days old pure culture of Sclerotium rolfsii. Each treatment was replicated three times with control.
The Petri plates with pathogen inoculated at one end alone, served as control. The Petri plates were then incubated at 28±2˚C. Three replications were maintained in each treatment. Per cent growth inhibition of mycelia growth over control was calculated by using the formula given by Vincent (1947):
I= (C-T)/C×100
Were,
I = Per cent inhibition in growth of test pathogen
C = Radial growth (mm) in control
T = Radial growth (mm) in treatment.
RESULT AND DISCUSSION
A set of eight oil cake extract including Neem seed cake, Groundnut cake, Castor cake, Soybean cake, Cotton cake, Mustard cake, Vermicompost and Farm yard manure were used to evaluate their efficacy in inhibiting S. rolfsii at 10 and 20 per cent concentration under in-vitro condition using poison food technique. The maximum per cent growth inhibition (68.18) in growth of S. rolfsii was recorded in Neem seed cake extract followed by Groundnut cake extract where 62.16 per cent inhibition was recorded after 72 hours of incubation period at 10 per cent concentration. However, at 20 per cent concentration, maximum inhibition of 77.77 per cent in growth of S. rolfsii was recorded by Neem seed cake extract after 72 hours of incubation. However, minimum inhibition of per cent was recorded in 20 per cent Farm yard manure extract.
After 120 hours of incubation, Maximum inhibition of 61.85 per cent in growth of S. rolfsii was recorded by 20 per cent Neem seed cake extract. However, minimum inhibition of 15.33 per cent was recorded in 20 per cent Farm yard manure extract. In this way, out of eight oil cake extracts tested, two oil cake extract namely Groundnut cake and Neem seed cake showed more than 50 per cent inhibition in growth of S. rolfsii at 20 per cent concentration after 120 hours of incubation period.
Similarly result reported by Dhingani et al. (2013) in which out of several organic extracts tested neem cake had the maximum mycelial growth inhibition (59.40%) followed by farm yard manure, castor cake and mustard cake. Also, Dubey et al. (2009) reported that autoclaved neem extracts had growth inhibition of 19.7, 30.6 and 42.3 per cent at one, five and ten percentage, respectively.
How to cite this article
Rakesh Gurjar, A.R. Wasnekar, Mahesh Kumar Mimrot, Yashowardhan Singh, Pushkar Dev and Jitendra Gurjar (2022). Management of Collor Rot of Chickpea caused by sclerotium rolfsii through oil Seed Cakes in vitro. Biological Forum – An International Journal, 14(2): 704-707.