The present investigation was conducted during the year 2022-2023 at the Department of Plant Pathology, N. M. College of Agriculture, NAU, Navsari with an aim to manage seed mycoflora of yardlong bean using seed treatment of different fungicides. The study also recorded the data regarding seed germination (%), root length, shoot length and seedling vigour index for different treatments. For in vitro experiment paper towel method was used while in vivo experiment was conducted using pot method. Under in vitro condition trifloxystrobin 25 + tebuconazole 50WG found to be best which recorded highest seed germination 73.00 per cent, shoot length 12.69 cm, root length 12.14 cm and seedling vigour index 1811.32 followed by zineb 68 + hexaconazole 4WP (66.50%, 12.64 cm, 7.33 cm and 1327.94). Under in vivo condition metalaxyl 8 + mancozeb 64WP recorded highest seed germination 70.63 per cent, shoot length 22.21 cm, root length 5.04 cm and seedling vigour index 1923.33 followed by carbendazim 12 + mancozeb 63WP (70.00%, 19.21 cm, 3.76 cm and 1607.27)

Seed mycoflora, yardlong bean, fungicides, shoot length, root length, seedling vigour index

A total of ten fungal species were isolated using three methods: the agar plate method, the standard blotter method, and the deep-freezing method. Yardlong bean seeds were treated with different fungicides under in vitro and in vivo cinditions to check their effect on seedling vigour index. Among the seven fungicidal seed treatments evaluated under in vitro condition, trifloxystrobin 25 + tebuconazole 50WG emerged as the most effective treatment. It recorded the highest values for seed germination (73.00%), shoot length (12.69cm), root length (12.14cm) and seedling vigour index (1811.32), followed by zineb 68% + hexaconazole 4WP, which showed 66.50 per cent germination, 12.64cm shoot length, 7.33cm root length, and a seedling vigour index of 1327.94.Under in vivo condition, metalaxyl 8 + mancozeb 64WP recorded the highest seed germination (70.63%), with a shoot length of 22.21cm, root length of 5.04cm and a seedling vigour index of 1923.33. This was followed by carbendazim 12 + mancozeb 63WP, which showed 70.00 per cent germination, 19.21 cm shoot length, 3.76 cm root length, and a seedling vigour index of 1607.27

S.A. Rathod, V.P. Prajapati, Snehal Patel, P.R. Patel and Alok Shrivastava (2025). Management of Seed Mycoflora of Yardlong Bean by Means of Seed Treatment with Fungicides In Vitro and In Vivo. Biological Forum, 17(7): 109-115