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Media Optimization for the Production of Tyrosinase Enzyme from Isolated Bacterial strain PV24

Author: Tungikar V.V. and Dixit P.P.

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Abstract

Using the Plackett-Burman design, a fermentation medium was created for the current study in order to maximize the synthesis of tyrosinase from the strain that was isolated from the Ahmednagar region. This was done by screening various nutritional and physical characteristics. CusO4, L-tyrosine, D-mannitol, and beef extract were the four fermentation process variables chosen from the One variable at a time (OVAT) method. Plackett Burman statistical analysis was used to assess the chosen variables based on standard effect plot, coefficient value, and significance. Three variables were shown to have a high degree of influence on production: D-mannitol, beef extract, and L-tyrosine. The other variable did not significantly affect production, according to the data. Additionally, the study of the variance value R2 (0.92) demonstrated the significance of the prediction model (p less than 0.05). Plotting the standard effect for every component and its characteristics gave precise information that may be used to choose appropriate variables for additional optimization. Tyrosinase activity was determined to be 49.9U/ml

Keywords

Tyrosinase, Bacteria, OVAT analysis, Enzyme assay, L- tyrosine, Plackett Burman Analysis, Media optimization, L-DOPA

Conclusion

The optimization of process parameters for the newly identified bacterial strain PV24, known for its robust tyrosinase activity, revealed significant findings. Mannitol and beef extract emerged as critical components, leading to an impressive peak tyrosinase activity of 49.9 U/ml. These results highlight the potential of this isolated bacterium as a valuable and reliable industrial source of tyrosinase

References

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How to cite this article

Tungikar V.V. and Dixit P.P. (2023). Media Optimization for the Production of Tyrosinase Enzyme from Isolated Bacterial strain PV24. Biological Forum – An International Journal, 15(4): 1028-1034