Medium Engineering for Micropropagation of Rose (Rosa hybrida L.) cv. First Red

Author: Pawan Kumar Sharma

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Abstract

In present study, the axillary buds were used to regenerate whole plant under in vitro conditions. Out of 23 media compositions supplemented with different combinations of plant growth regulators, only 10 medium concentrations responded and MS basal medium with BAP 4mg/l and GA3 3mg/l was best with 70% bud break after 5 weeks of initial culturing. Releases of phenols were checked through addition of ascorbic acid @ 200 mg/l in combination with PVP (100mg/l). Finally MS medium supplemented with TDZ (0.5mg/l) in addition to antioxidants, totally controlled yellowing & senescence. On an average 9 shoots were obtained on this medium with average shoot length of 2.37 cm after every four weeks. 22°C was the most congenial temperature for shoot multiplication. Stem tissue of about 2cm gave average eight shoots while only an average of 2 shoots were obtained when the size was 0.5cm. Additionally, it was observed that rate of bud break was much higher when explants were collected after 40 days

Keywords

Axiilary buds, in vitro propagation, Murashige and Skoog medium, shooting, rooting.

Conclusion

A fully reproducible protocol for in vitro propagation of rose (Rosa hybrida L.)cv. First Red has been developed using axillary buds as explants. Rose is an important ornamental plant which needs to be multiplied at a fast rate to fulfill the demands of floriculture industry. In the present investigations various obstacles were met at different stages of micro propagation. These were one by one overcome by following/ modifying different cultural conditions and practices.

References

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How to cite this article

Pawan Kumar Sharma (2017). Medium Engineering for Micropropagation of Rose (Rosa hybrida L.) cv. First Red , Biological Forum – An International Journal 9(1): 26-30.