Molecular Characterization of Doubled Haploid Rice Lines Derived from the F1s of IR20 × Mahulata

Author: Sandeep Kumar Singh*, Jawahar Lal Katara, Prem Narayan Jagadev, Kishor Jeughale, Sanghamitra Samantaray, Debendra Nath Bastia, Parameswaran C., Manjusha Chandravani and Suryasmita Mallick

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Abstract

Characterization and variability evaluation is vital for the development of crop plant. Majority of the research on DH lines has focused on evaluating their overall agronomic performance in comparison to original genotype and/or lines developed through traditional breeding. However, this experiment focused to assess the molecular variation of rice (Oryza sativa L.) doubled haploid lines. DNA markers, specifically Simple Sequence Repeats (SSR) is a powerful tool for the evaluation of genetic variations and resolving cultivar identities. An overall of sixty-four doubled haploid rice lines derived from the F1s of IR20 × Mahulata through anther culture alongside its parents have been genotyped and clustered by using SSR markers markers. 95 SSR markers, out of 490 SSR markers have been found to be informative among the parents, have been utilized in genotyping the entire population. Based on dendrogram clustering, the DH-lines have been grouped into 3 clusters viz. cluster-I, cluster-II an

Keywords

Doubled haploids, Rice, SSR marker, Clustering, Variability, Genetic diversity.

Conclusion

Through hybridization, genetic diversity has a direct relationship to the creation of variability. The DH-lines were divided into three major clusters in this study: cluster I, cluster II, and cluster III. To create variability and generate heterotic cross pairs for generating hybrid vigour, as well as for use in the hybridization programme, genotypes from different clusters should be employed rather than genotypes from clusters with low divergence (Narayanan and Murugan, 2013). Parents with a high yield potential and a large genetic variety are more likely to produce superior transgressive segregants in a small space of time in breeding programmes (Maurya and Singh, 1977). These genotypes can also be used in heterosis breeding to create recombinants with a high yield (Mohanty et al., 2020). Furthermore, the effectiveness of identifying transgressive segregants or recombinants can be improved by using marker systems as a biotechnological tool. Higher molecular variability will be ident

References

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How to cite this article

Sandeep Kumar Singh, Jawahar Lal Katara, Prem Narayan Jagadev, Kishor Jeughale, Sanghamitra Samantaray, Debendra Nath Bastia, Parameswaran C., Manjusha Chandravani and Suryasmita Mallick (2022). Molecular Characterization of Doubled Haploid Rice Lines Der