Molecular Detection and Partial Characterization of Begomovirus Associated Yellow Mosaic virus Disease of Soyabean (Glycine max L.)

Author: Y.B. Naveesh, H.A. Prameela, N. Sagar, S. Basavaraj, N. Nagaraju and K.T. Rangaswamy

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Abstract

The study's primary aim was to explore the molecular connection between the virus responsible for soybean yellow mosaic disease and related viruses. Symptoms of the disease involve yellowing leaves, leaf rolling and stunted growth in infected plants. Specific degenerate primers for begomoviruses were utilized to detect the causative virus. Gene fragments, measuring 1000bp for the coat protein and 900bp for the movement protein, were amplified from both naturally infected and whitefly-inoculated plants. Comparative analysis of the virus's CP gene sequence with other begomoviruses showcased clustering with horse gram yellow mosaic virus (HgYMV) segments from a horse gram isolate and an HgYMV isolate from India, Bangalore, infecting French beans, sharing a 98 per cent similarity, respectively. Similarly, when assessing the virus's MP gene sequence against other begomoviruses, it clustered with Horsegram yellow mosaic virus segments from a horse gram isolate and the HgYMV isolate from India, Bangalore, which infects French beans, showing a 99 per cent similarity, respectively. Consequently, the findings distinctly reveal that the yellow mosaic virus infecting soybeans is closely related to the one infecting horse gram and French beans, leading to its recognition as a strain of horse gram yellow mosaic virus (HgYMV).

Keywords

Soyabean, Begomovirus, PCR, coat protein, movement protein, Molecular Characterization

Conclusion

The scrutiny of soybean yellow mosaic virus (SYMV) and its relation to other begomoviruses offers crucial insights into their genetic diversity and classification. The study highlighted the close genetic kinship between SYMV isolates from Bengaluru and Horse gram yellow mosaic virus (HgYMV), especially evident in the coat protein (CP) and movement protein (MP) gene sequences. This distinction underscores SYMV's unique nature and its divergence from other legume-affecting yellow mosaic viruses. Additionally, the presence of diverse begomovirus groups with their unique genetic profiles emphasizes the role of recombination in generating their variability. These revelations deepen our comprehension of begomovirus genetic diversity and their impact on soybean farming, notably in areas afflicted by yellow mosaic disease.

References

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How to cite this article

Y.B. Naveesh, H.A. Prameela, N. Sagar, S. Basavaraj, N. Nagaraju and K.T. Rangaswamy (2023). Molecular Detection and Partial characterization of begomovirus Associated Yellow Mosaic virus Disease of Soyabean (Glycine max L.). Biological Forum – An International Journal, 15(10): 965-969.