Molecular Identification and Evaluation of different Chemicals and Bioagents against Pomegranate Wilt caused by Ceratocystis fimbriata under Greenhouse condition

Author: G. Abhishek, G. Manjunatha*, R.K. Mesta, R. Gunnaiah, D.R. Patil and R. Suma

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Abstract

A lab and greenhouse experiment was conducted at the College of Horticulture, Bagalkot, Karnataka, during 2019 for molecular identification of Ceratocystis fimbriata and to evaluate chemicals and bioagents against pomegranate wilt. Pomegranates are susceptible to a variety of serious diseases, the most devastating of which is pomegranate wilt caused by C. fimbriata. It is critical to identify and manage C. fimbriata accurately and quickly. Results revealed that designed primer produced or amplified a single band of 268 bp in fungal DNA and infected root sample but not in healthy root sample. Under greenhouse condition data obtained 120 days after drenching revealed that propiconazole @ 0.2% recorded 22.77 % of branches infected which was on par with tebuconazole @ 0.2% which recorded 24.03 % of branches infected. This result help for accurate identification of pathogen and to develop different IDM modules for management of pomegranate wilt under filed condition.

Keywords

Pomegranate, Pomegranate wilt, Ceratocystis fimbriata, PCR, Molecular identification, Chemical management, Bioagents.

Conclusion

The PCR assay developed is precise, rapid and consistent. Therefore, for the study of pathogen biology and host-pathogen interactions, this assay will be very useful and gain immense perception to address underlying problems with pomegranate wilt complex. This could be a useful tool for early-stage pomegranate wilt identification and diagnosis. Considering all of these findings and facts, it is strongly recommended that the PCR assay be used as an efficient and dependable tool for evaluating pomegranate plants. Greenhouse evaluation of different fungicides and bioagents provides primary results and information regarding their efficacy against pomegranate wilt caused by C. fimbriata and with an idea to utilize these promising fungicides and bioagents and combination as different modules for management of pomegranate wilt under field conditions.

References

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How to cite this article

G. Abhishek, G. Manjunatha, R.K. Mesta, R. Gunnaiah, D.R. Patil and R. Suma (2022). Molecular identification and evaluation of different chemicals and bioagents against pomegranate wilt caused by Ceratocystis fimbriata under greenhouse condition. Biologic