The NAA (1-naphthalene acetic acid) is an important auxin, commonly utilize in plant tissue culture for micropropagation of various plant species. The degree and direction of regeneration response of explant, highly influences with the concentration of growth regulator. Therefore, in the present investigation, effects of different levels of NAA (0.5 to 4.0 mg/l) were evaluated for shoot proliferation, callus induction and root induction of Aloe vera. Various concentrations of NAA had shown differential effects on lateral shoot explant in MS (Murashige and Skoog) medium. The treatment of 2.5 mg/l induced highest number of shoots (3.10) per explant, followed by 3.0 mg/l NAA (2.90) with 80 per cent induction frequency. Maximum callus weight (1.93 g) was observed at 3.5 mg/l with 50 per cent frequency while, highest number of roots (5.0) was obtained at 1.5 mg/l with root induction frequency of 90 per cent. Thus, for best economical use, these identified levels of NAA can be utilized for g

Aloe, Callus, Micropropagation, NAA, Root induction, Shoot proliferation.

Based on our study it was found that, NAA in MS medium showed all rounder effects on lateral shoot explant under in vitro condition. It can be utilized for shoot proliferation at intermediate levels (2.0-3.0 mg/l), callus induction at higher levels (more than 3.0 mg/l) and root induction at lower levels (below 2.0 mg/l). Therefore, for best economical use, identified levels of NAA can be utilized for getting maximum regeneration response of Aloe under in vitro condition.

Sarfraz Ahmad, M.L. Jakhar, S.S. Rajput, S.S. Punia, Varsha Kumari and M.P. Ola (2022). Morphogenetic Effect of NAA (1-naphthalene acetic acid) on in-vitro Regeneration of Aloe vera. Biological Forum – An International Journal, 14(1): 451-454.