Morphological and Molecular Evaluation of Potential Indigenous Grain Amaranth (Amaranthus hypochondriacus L.) Genotypes of India

Author: R.S. Solanki, N.N. Prajapati, D.P. Joshi, K.K. Tiwari and P.M. Rahevar

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Abstract

The presence of genetic variation and its maintenance are essential components in plant breeding. Considering these, ten phenotypic characters, one biochemical component and seven microsatellite primers were employed to investigate the genetic diversity in forty-six grain amaranth genotypes. The values of GCV were lower than that of PCV for each individual character under investigation. Seed yield plant-1 (g) demonstrated a strong genotypic level association with days to flowering, leaf area plant-1(cm2), length of inflorescence (cm), plant height (cm) and harvest index (%). The path coefficient study revealed that harvest index (%) had the strongest direct positive impact on seed yield plant-1 (g). Seven ISSR primers resulted in a total of 46 DNA amplicons, from which 40 were polymorphic. The data obtained from all markers and related amplicons classified the genotypes into six UPGMA based clusters. Among the grain amaranth genotypes RGA-16 and RGA-17 evident maximum similarity; while the genotypes BGA-10 and BGA-4-9 shown minimum similarity on the basis of Jaccard’s co-efficient result. Breeding of new cultivar using this varieties will helpful in the crop improvement programmes. The results of the present investigation concluded that to obtain actual genetic diversity, the molecular and morphological markers should be used in tandem.

Keywords

DNA amplicons, Genetic variation, Grain amaranth, ISSR, Path co-efficient

Conclusion

While imposing selection for genetic improvement of grain amaranth, due weightage should be given on days to flowering, length of inflorescence (cm), leaf area plant-1(cm2) plant height (cm) and harvest index (%). Also, ISSR markers used in this study appeared to be worthy for the molecular assessment and evaluating the genetic relationship among the genotypes of grain amaranth.

References

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How to cite this article

R.S. Solanki, N.N. Prajapati, D.P. Joshi, K.K. Tiwari and P.M. Rahevar (2023). Morphological and Molecular Evaluation of Potential Indigenous Grain Amaranth (Amaranthus hypochondriacus L.) Genotypes of India. Biological Forum – An International Journal, 15(4): 620-625.