Morphological Diversity and Isolation of Genomic DNA of Garcinia pedunculata (Roxburgh ex Buchanan-Hamilton) from selected districts of Brahmaputra Valley of Assam, India

Author: Himadree Phukon, Sarojmoni Sonowal, Mridusmita Dutta, Tridisha Borgohain, Gunjan Jain and Inkita Goswami

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Abstract

In the present study, the morphological features and isolation of genomic DNA of the Garcinia pedunculata was conducted from five sub-sites each from three Districts namely Dibrugarh, Sivasagar and Tinsukia from Brahmaputra valley of upper Assam for a period of one year, from June 2014 to May 2015. Morphological characterization were studied for mature and bearing trees with respect to leaf size, size of leaf petiole, length of leaf vein, size of petiole and pedicels, fruit size etc. Extraction and isolation of genomic DNA from leaf tissue was done by Modified Doyle & Doyle (1990) methodology using CTAB extraction buffer. Results of the study reveals that the morphological features of the Garcinia pedunculata from different geographical locations showed a significant variation, particularly for leaf and fruit characteristics. Isolation and purification of genomic DNA from leaf tissue by Modified Doyle & Doyle (1990) methodology using CTAB extraction buffer indicates that the isolated genomic DNA may have more than 5000 bp. Isolation and purification of genomic DNA by using Modified Doyle & Doyle (1990) methodology using CTAB extraction buffer is a reliable measurement of DNA concentration and its purity. The research involve collecting plant samples from various districts, documenting and analyzing their morphological traits, and then isolating the genomic DNA from these samples. Due to limited access to specialized equipment or consumables necessary for the CTAB method, the extraction process was hindered.

Keywords

Assam, Brahmaputra Valley, Garcinia pedunculata, Genomic DNA, Morphological diversity

Conclusion

All molecular and genetic studies rely on accuracy in measurement of DNA concentration and purity. Morphological features of the Garcinia pedunculata from different geographical locations showed a significant variation, particularly for leaf and fruit characteristics. Isolation and purification of genomic DNA from leaf tissue by Modified Doyle & Doyle (1990) methodology using CTAB extraction buffer indicates that the isolated genomic DNA may have more than 5000 bp. Sahasrabudhe et al. (2010), worked on standardization of DNA extraction and optimization of RAPD-PCR conditions of Garcinia indica. They worked on modified CTAB protocol adding polyvinylpyrrolidone (PVP) in separate tubes and precipitating with 5M Nacl along with chilled alcohol to increase the solubility of polysaccharides. They could isolate pure and sufficient amount of DNA and optimize RAPD conditions. Isolation and purification of genomic DNA by using Modified Doyle & Doyle (1990) methodology using CTAB extraction buffer is a reliable measurement of DNA concentration and its purity. This study will act as a tool for other researchers in this field to continue their work further on Garcinia species and understand the morphological diversity. Such studies will help in authentication of the genus Garcinia.

References

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How to cite this article

Himadree Phukon, Sarojmoni Sonowal, Mridusmita Dutta, Tridisha Borgohain, Gunjan Jain and Inkita Goswami (2023). Morphological Diversity and Isolation of Genomic DNA of Garcinia pedunculata (Roxburgh ex Buchanan-Hamilton) from selected districts of Brahmaputra Valley of Assam, India. Biological Forum – An International Journal, 15(5): 1627-1633.