Mutagenic Effectiveness and Efficiency of Chemical Mutagens in Barnyard millet (Echinochloa frumentacea)

Author: M. Karthikeyan*, C. Vanniarajan, M. Vetriventhan, A. Nirmalakumari, R. Shanmuga Sundaram, T. Sivakumar and G. Srinivasan

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Abstract

Mutagenic effectiveness and efficiency of individual treatments of Ethyl Methane Sulphonate (EMS) and Sodium Azide (SA) were assessed in Barnyard millet. The treatments included three doses each of EMS and SA calculated based on their Lethal Dose (LD50). Different types of chlorophyll mutants namely albino, xantha, chlorina, striata, alboviridis, xanthoviridis and albomaculata were observed in the M2 population. Based on plant survival and chlorophyll mutation frequency in M2 generation, the effectiveness and efficiency were calculated for both the chemical mutagens. Mutagenic effectiveness and efficiency increased with the decrease in the concentration of mutagen. Hence, lower doses of chemical mutagens have proved to be more effective and beneficial in inducing mutations in barnyard millet.

Keywords

Barnyard millet, Chemical mutagens, Mutagenic effectiveness and efficiency, Mutation breeding, Ethyl Methane Sulphonate, Sodium Azide.

Conclusion

Mutagenic effectiveness and efficiency of individual treatments of Ethyl Methane Sulphonate (EMS) and Sodium Azide (SA) were assessed in Barnyard millet. The treatments included three doses each of EMS and SA calculated based on their Lethal Dose (LD50). Different types of chlorophyll mutants namely albino, xantha, chlorina, striata, alboviridis, xanthoviridis and albomaculata were observed in the M2 population. Based on plant survival and chlorophyll mutation frequency in M2 generation, the effectiveness and efficiency were calculated for both the chemical mutagens. Mutagenic effectiveness and efficiency increased with the decrease in the concentration of mutagen. Hence, lower doses of chemical mutagens have proved to be more effective and beneficial in inducing mutations in barnyard millet.

References

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How to cite this article

M. Karthikeyan, C. Vanniarajan, M. Vetriventhan, A. Nirmalakumari, R. Shanmuga Sundaram, T. Sivakumar and G. Srinivasan (2022). Mutagenic Effectiveness and Efficiency of Chemical Mutagens in Barnyard millet (Echinochloa frumentacea). Biological Forum –