The aim of this study is to evaluate the effect of different concentrations of citral on glutathione (GSH), nitric oxide (NO), lipid peroxide (LPO) and vitamin C. Also, the protective effect of N. sativa was studied. Fertilized eggs of the chick Gallus domesticus were divided into control or experimental groups which received three different concentrations of citral (50, 100 and 200 μM), N. sativa extract (5 μl) or a combination of N. sativa extract with citral. Citral and N. sativa groups decreased GSH & vitamin C and NO, while increased LPO. Co-treatment with citral and N. sativa increased GSH & vitamin C and decreased LPO levels. Citral induced oxidative stress by inhibiting RA synthesis. The limited mitigative properties of N. sativa are attributed to either its antineoplastic properties, the high levels of oxidative stress provoked by citral and above its antioxidative capacity or the low administered dose.

Citral, Nigella sativa, GSH, NO, LPO, Vitamin C, Chick embryo.

The aim of this study is to evaluate the effect of different concentrations of citral on glutathione (GSH), nitric oxide (NO), lipid peroxide (LPO) and vitamin C. Also, the protective effect of N. sativa was studied. Fertilized eggs of the chick Gallus domesticus were divided into control or experimental groups which received three different concentrations of citral (50, 100 and 200 μM), N. sativa extract (5 μl) or a combination of N. sativa extract with citral. Citral and N. sativa groups decreased GSH & vitamin C and NO, while increased LPO. Co-treatment with citral and N. sativa increased GSH & vitamin C and decreased LPO levels. Citral induced oxidative stress by inhibiting RA synthesis. The limited mitigative properties of N. sativa are attributed to either its antineoplastic properties, the high levels of oxidative stress provoked by citral and above its antioxidative capacity or the low administered dose.

Reda A. Ali, Hanem S. Abdel-Tawab and Dalia Elzahraa F. Mostafa (2018). Nigella sativa Mitigative Antioxidant Properties against Citral induced Oxidative Stress and Biochemical changes during Development of Chick Embryo. Biological Forum – An Internatio