DNA barcoding categorized the plant materials based on short, standardized gene sequence in precise manner. Four plant DNA barcode markers called rbcL, matK, trnHpsbA, and ITS2 have been developed for making revolutionary advancements in the field of ecology, evolutionary biology and conservation, including community assembly, species interaction networks, natural conservation, taxonomic discovery, and assessing priority areas for environmental protection and also potential applications in the field of forensics. DNA barcoding uses particular regions of DNA making helping in categorization and recognizing unidentified species. Researchers now interested to generate DNA barcodes designed for all living organisms and to build up data accessible to public to help in understanding of natural biodiversity of world. Cyclotides are peptides derived from plants with particular head to tail cyclic backbone that have three disulphide bonds by forming a cystine knot. Recent information about DNA

DNA barcoding, Genomic Sequencing, Cyclotides, Evolutionary biology, Genetic Markers.

The achievement goals of sequencing technology such as operation of micro fluidic PCR basis with improvement in traditional techniques with the intention to offer a quicker and less exclusive option at huge scale multi locus on plant DNA barcoding are diagnostic tool of current state of discoveries in genomics. Studies on clinical activities of cyclotides needed more experimentation and research on clinical projects. Various cyclotides showed that they are orally active except small literature about their oral activity.

Naeem, M., Ali, J., Hassan, M.Z., Arshad, B., Rao, M.H.I., Sarmad, M.S.K., Irfan, U., Khan, N.A., Sohail, M.S., Umar, M. & Hassan, M.U. (2019). Novel Approach Towards DNA Barcoding as a Tool in Molecular Biology and Biological Activities of Cyclotides