Optimization of various Physiochemical Parameters to Enhance 2- Naphthalene Sulfonate Biodegradation by an indigenous Bacterial isolate C2

Author: Prince Dhammi and Harvinder Singh Saini

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Abstract

2-Naphthalene sulfonate (2-NS), a textile dye intermediate, is highly toxic and has limited biodegradability which poses a threat to aquatic life and human health. Enrichment of sludge from treatment plant receiving textile effluent resulted in isolation of 2-NS degrading bacteria, designated as Isolate C2. To achieve maximum biodegradation activity, optimization of different factors such as growth media, 2-NS concentrations, pH, incubation temperature, inoculum level, and time interval was performed using one factor at a time approach. The best degradation ability (100%) was obtained in 8h, when cells were grown in Sulfur free Mineral Salt Medium using OD540 1.0, pH 7 at 30°C. Isolate C2 was versatile enough to show growth and degradation at 2-NS concentrations ranging from 25-200 mg/L. In nutshell, the study revealed that 2-NS was efficiently consumed by C2 as the sole carbon source and therefore is as an important tool in the bioremediation of wastewater effluent.

Keywords

Biodegradation, Optimization, 2- Naphthalene sulfonate, textile effluent, textile dye intermediate, degrading bacteria, physiochemical parameters

Conclusion

This study thus highlights the significant effect of various physiochemical parameters on the degradation potential of isolate C2. The isolate exhibited an enhanced degradative capacity (100 mg/L of 2-NS in 8h) under these optimized conditions and was able to use 2-Naphthalene sulfonate as a sole source of carbon and energy. Further, the HPLC analysis of transformed sample (at 8h) revealed the complete degradation of 2-Naphthalene sulfonate. Therefore, the study concludes that the polluted sites could act as a source for isolation of indigenous potential degraders which could assist the scientific community to tackle the environmental haphazard created by such persistent organic pollutants.

References

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How to cite this article

Prince Dhammi and Harvinder Singh Saini (2023). Optimization of various Physiochemical Parameters to Enhance 2- Naphthalene Sulfonate Biodegradation by an indigenous Bacterial isolate C2. Biological Forum – An International Journal, 15(5): 1598-1604.