Indexing

Phenotypic Screening and Molecular characterization for Leaf Rust Resistance Gene Lr9 in Wheat

Author: Elangbam Premabati Devi*, K.P. Singh, Deepshikha and J.P. Jaiswal

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Abstract

Field screening during both the experimental years of 2014-15 and 2015-16 has revealed that four varieties such as HW 2021, HP 1731, WH 896 and UP2565 were found as no infection of leaf rust at all, so they were considered as immune to disease. While five varieties such as WL1562, Chakwal 86, Rawal87, WH 1105, C306 were identified with disease severity ranged from 20 to 60% severity of susceptible response, so these varieties were identified as highly susceptible to leaf rust. The other remaining 15 varieties were recorded as disease severity ranged from TR to maximum of 10% of resistant response, so these varieties were considered as resistant to leaf rust. The result of AUDPC value has revealed that the maximum value of 880.25 was recorded in WH1105 followed by WL1562 with 540.4 values so they were considered as highly susceptible varieties. The maximum rate of infection was recorded in C306 with 0.382 followed by HD2189 and UP2572 with 0.249 values. Confirmation of presence of Lr9 g

Keywords

Leaf rust, SCAR marker, Lr9, resistant, AUDPC.

Conclusion

From the above findings it can be concluded that four varieties viz. HD2189, UP2572, UP2748 and WH896 were identified as promising varieties showing great resistant at both phenotypic and genotypic level linked with Lr9 gene against leaf rust of wheat. Moreover, some wheat varieties were showing resistant to leaf rust in field conditions but not detected with presence of Lr9 gene which may be due to presence of some other Lr genes. Thus, proper field evaluation of resistant vareities in high disease pressure along with the application of molecular marker could be an important challenging area in identifying resistant sources of varieties for enhancing rust resistance programme to combat wheat rust.

References

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How to cite this article

Elangbam Premabati Devi, K.P. Singh, Deepshikha and J.P. Jaiswal (2022). Phenotypic Screening and Molecular characterization for Leaf Rust Resistance Gene Lr9 in Wheat. Biological Forum – An International Journal, 14(1): 269-274.