Phylogenetic Exploration of Acacia nilotica: A Commonly used Medicinal Plant in Himachal Pradesh

Author: Deepika Rana

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Abstract

Acacia nilotica belongs to family, Fabaceae. This family is known to contain highest number of species. The genetic diversity and relationship of six samples of Acacia nilotica collected from different geographical regions of Himachal Pradesh, was studied. The regions selected for the analysis were: Solan, Sirmour, Mandi, Bilaspur, Una and Hamirpur. Using RAPD analysis, a total of 12 arbitrary primers were screened while 7 primers showed amplifications. Out of total 68 bands, total 7 monomorphic and 61 polymorphic bands were scored. The percentage of polymorphism shown by the primers was 89%. The genetic diversity was estimated by constructing dendrogram using UPGMA method. The six samples of Acacia nilotica were divided into two major clusters. The genotypes of Mandi district showed 44% similarity with the genotypes of Bilaspur district. The genetic similarity between genotypes of Sirmour, Mandi, Bilaspur, Una was 30.2%. The present study revealed that the variation exists among the d

Keywords

Acacia nilotica, RAPD, genetic variation, polymorphism.

Conclusion

A. nilotica have certain important secondary metabolites that confer different pharmacological activities, which can be useful for mankind. This tree is perennial in nature and that may be the reason for this type of variations. For the conservation of a particular plant species the accurate assessment of genetic diversity is an important objective. The present study might be helpful in the field of genetic variability in A. nilotica. As results of the present study concluded that A. nilotica collected from different geographical areas of Himachal Pradesh possess a certain level of genetic diversity. This study indicated that RAPD is sufficiently informative and powerful technique to detect genetic variability in natural populations of A. nilotica. From this study it was concluded that geographical diversity is important and associated with the sole factor of determining the genetic diversity in A. nilotica.

References

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How to cite this article

Deepika Rana (2018). Phylogenetic Exploration of Acacia nilotica: A Commonly used Medicinal Plant in Himachal Pradesh, Biological Forum – An International Journal 10(1): 69-74.