Urolithiasis plaques are a common urinary disease in all over the world which is defined as one or more calculi present in the urinary tract. The prevalence rate and incidence are increasing now - a - days. In Siddha medicines many formulations have been used in the treatment for Urolithiasis. Amurthathi Chooranam is one among the Siddha poly herbal formulation which is indicated for Urolithiasis. The aim of the study is to evaluate the physico-chemical, phyto-chemical and sophisticated analysis of Siddha poly herbal formulation “Amurthathi Chooranam”. Amurthathi Chooranam was prepared as per the Siddha literature Anubava Vaithiya Deva Ragasiyam. Physicochemical analysis such as water soluble extract 24.9985%, Alcohol soluble extract 5.1505%, Loss on drying 3.598%, Total ash 7.3816%, Acid insoluble Ash 3.0436%, Water soluble ash 1.2543%, phytochemical such as Alkaloids, carbohydrates, tannin, phenol, saponin, flavonoids and diterpenes are present. Other parameters like HPTLC, microbial load, specific pathogen, pesticide residues and aflatoxins were evaluated as per PLIM guidelines. From the outcome of results the drug Amurthathi Chooranam is safe, cost effective and biologically having active components which is treating for urolithiasis. This evidence based data is giving global acceptance of purity, in a prompt manner regarding Amurthathi Chooranam

Amuthathi Chooranam, Siddha formulation, Physicochemical, Phytochemical screening

Over a number of decades, urolithiasis has become the most important threats in human health globally, because of the lack of effective treatments, greater recurrence rates and it’s multiple etiology factors. Standardization is the most powerful but we are least using tools. Documentation of the above results and discussions confirms that the Amurthathi Chooranam has potent drug indicated for urolithiasis. Physicochemical analysis such as water soluble extract 24.9985%, Alcohol soluble extract 5.1505%, Loss on drying 3.598%, Total ash 7.3816%, Acid insoluble Ash 3.0436%, Water soluble ash 1.2543%, which reveals that the drug has good quality and purity. It specifies no impurities in the raw drug A MC. The stability and shelf life of the drug AMC is good so very safe to treat urolithiasis. Powder microscopic analysis strengthens the drug has no adulterations and there are no impurities. Phytochemical such as Alkaloids, carbohydrates, tannin, phenol, saponin, flavonoids and diterpenes are having reduction of super saturation of lithogenic enhancing properties. Other parameters like HPTLC, demonstrates a chemical fingerprint for authentication and reliable verification of the existence of bioactive compounds in AMC. So, the presence of important phytochemicals in AMC was strengthened by TLC and comparing the Rf of corresponding spot with that of standards. The drug AMC possesses anti-urolithiatic property. Sterility test reveals, AMC is free from microorganisms. So the drug has good quality and safety. A specific pathogen test showed that the drug AMC prevents the growth of microorganisms which shows that the drug AMC can be used to decrease the morbidity and mortality from chronic diseases. The aflatoxin assay results revealed that the drug AMC is non-toxic, no contamination and it does not acts as a carcinogen. The pesticide precipitate test demonstrated that the drug AMC has no toxicity and bioaccumulation. Hence, the drug AMC is safe to treat urolithiasis. From the outcome of results the drug Amurthathi Chooranam is safe and cost effective and biologically having active components which is treating for urolithiasis

Ramani Mani, Kabilan Natarajan and Kanakavalli Kadarkarai (2023). Physico-Chemical and Phytochemical Screening of Siddha Poly Herbal Formulation - Amurthathi Chooranam. Biological Forum – An International Journal, 15(3a): 69-79