Plant Growth Promotion and Nif H Gene Amplification of Bacteria Isolated From Different Agro-Climatic Zones of Odisha

Author: Sujata Priyadarshini*, Aiswarya Panda and Bibhuti Bhusan Mishra

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Abstract

Indiscriminate use of chemical fertilizers has led to environmental contamination including deterioration of soil health and nitrogen status. Hence it is imperative to inculcate biological agents in order to reduce the ill effects of chemical fertilizer. Use of potent bacteria as bio-fertilizer can improve the soil nitrogen status leading to improved soil health and increased productivity. From the rhizospheric soil of different agro-climatic zones of Odisha, 63 bacteria were isolated and their plant growth promoting traits were studied. Sixteen potent bacteria showing maximum PGP traits were subjected to RT-PCR for nif H gene quantification to ascertain biological nitrification. Two isolates BS 2 and BS45 showing highest copy number increased growth performance viz. shoot length, root length, fresh & dry biomass and no. of leaves of green gram, black gram and pigeon pea. After the 16s r-DNA sequencing BS2 and BS45, were identified as Klebsiella quasipneumoniae and Enterobacter roggenk

Keywords

Acid Soil, Agroclimatic Zone, Growth of Pulses, Nif H Amplification, Plant Growth Promoting Traits.