Preliminary Phytochemical Profiles and Antioxidant Activities of Methanolic, Chloroform and Hexane Extracts of Alpinia galangal Rhizome and Morshella esculenta Mycelia

Author: Nilanjana Prasad and Kashish Gupta

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Abstract

Alpinia galangal and Morshella esculenta are ethnomedicinal plants due to their radical scavenging and gastronomic qualities. This prompted us to carry out this study about the antioxidant activity of plants. Extraction was carried out using solvents by maceration followed by phytochemical screening. Purification of crude was done by silica gel column chromatography. Total phenolic and flavonoid content (TPC) and (TFC) of crude and fractions were determined. The antioxidant activities were assessed by scavenging assays. TPC of methanolic extract of A. galangal was found highest with the value of 49.787±0.063 GAE mg/g and showed higher TFC with the value of 38.013±2.116 QCE mg/g. The highest H2O2 activity was shown by fraction MEC5 (83.363%). The highest ABTS radical scavenging activity was shown by AGC (99.68%). However, our study faced different challenges such as differences between antioxidant activities, H2O2 activity etc. In conclusion, three different solvent extracts of A. galangal and M. esculenta revealed the presence or absence of phytochemicals tested and varying degrees of in-vitro antioxidant activity.

Keywords

Alpinia galangal, Morshella esculenta, phytochemicals, antioxidant, hydrogen peroxide

Conclusion

From the above study, it can be concluded that three different solvent extracts of methanol, chloroform and hexane of A. galangal rhizome and M. esculenta mycelia showed the presence and absence of phytochemicals tested and varying degrees of in-vitro ABTS and H2O2 antioxidant activities. Ethnomedicinal plants A. galangal and M. esculenta possessed antioxidant potential due to the presence of principal antioxidants such as phenols and flavonoids. Methanolic extracts of A. galangal rhizome rose out to be the profound hub for antioxidant activities because of their high TPC, TFC and ABTS radical scavenging potential. M. esculenta mycelia might be a promising herb due to its increased H2O2 radical scavenging activity. We are blessed with a plethora of magical natural ingredients from nature, it solely depends upon us to scientifically explore them for societal benefits.

References

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How to cite this article

Nilanjana Prasad and Kashish Gupta (2023). Preliminary Phytochemical Profiles and Antioxidant Activities of Methanolic, Chloroform and Hexane Extracts of Alpinia galangal Rhizome and Morshella esculenta Mycelia. Biological Forum – An International Journal, 15(4): 550-556.