Standardization of Siddha medicines is an important one to know the quality of drugs. Avuri Karpam (AK) is a poly herbal formulation consists of seven ingredients which have mentioned in classical Siddha literature “Pathartha Gunavilakkam” for treating Keelvatham, Udhirakkattu, Sarpavisham. This present study was intended to determine the organoleptic characters, Physico chemical testing, preliminary phytochemical analysis, powder microscopy, Thin layer chromatographic (TLC) and High-performance thin layer chromatographic (HPTLC) analysis. Plant materials were identified and authenticated by the experts. Purification and preparation process were done as per classical Siddha literature. All the parameters were analyzed by standard protocols. The results revealed the organoleptic characters, parameters of physico-chemical analysis, and the presence of phyto chemicals such as alkaloids, carbohydrates, saponin, phenols, tannin, flavanoids and diterpenes. The HPTLC fingerprint analysis revealed sixteen bioactive compounds when scanned at 520 nm, seventeen bioactive compounds when scanned at 366 nm, thirteen bioactive compounds when scanned at 254 nm. Powder microscopy documentation shows the authenticity of the formulation Avuri Karpam. From the results Avuri Karpam was proved that it has various potent medicinal properties over the defined standardization method. This research work will be used for further Pre-clinical and clinical studies

Avuri Karpam, Herbal drug standardization, Siddha drug, Phytochemical analysis, Chromatographic studies, Powder microscopy

From the results Avuri karpam was proved that it has various potent biological active properties over the defined standardization method and it may act therapeutically in treating various diseases. This research work will be used for further pre-clinical and clinical studies

T. Giftillda Selva Elsee, E.M. Manikgantan and G. Bharath Kumar (2023). Preliminary Standardization of Siddha Formulation avuri Karpam. Biological Forum – An International Journal, 15(3a): 89-96