Prevalence and Detection of viruses Associated with Mosaic Disease of Sugarcane in Andhra Pradesh

Author: G. Vamsi Krishna, V. Manoj Kumar, P. Kishore Varma, B. Bhavani and G. Vijaya Kumar

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Abstract

Sugarcane represents third largest crop in terms of value next to rice and wheat in India and it is one of the most important commercial crops cultivated worldwide for products like white sugar, bagasse and ethanol. Among the diseases of sugarcane, mosaic disease is one of the most destructive viral disease which imposes serious threat to sugarcane cultivation by reducing both sugar content and cane yield. Since diagnosis is very crucial for effective management of the disease, a comprehensive and systematic survey was conducted in 126 fields belonging to 126 villages spread over 14 districts of Andhra Pradesh, India for assessing the incidence of mosaic disease. Mosaic incidence varied across the districts in the range of 14 % to 90 % in commercial varieties. Highest mosaic incidence was observed in Anakapalle district (66.64 per cent (%)) followed by Krishna district (64.66 %) and least incidence was observed in Alluri Sitharamaraju district (17.08 %) followed by Parvathipuram Manyam district (31.77 %). Almost all the varieties grown in Andhra Pradesh viz., 2009A 107, 87A 298, 2003V 46, Co 86032, 81V 48, 2015A 311, Si-2010-123, Si-2010-309, ROC-16 and VCF-517 were affected, but percent incidence varied from genotype to genotype, location, stage of the crop and type of the crop. Symptoms varied from cultivar to cultivar. Symptomatic sugarcane leaf samples were collected, total RNA was isolated from infected samples and RT-PCR assays were performed using Sugarcane mosaic virus and Sugarcane streak mosaic virus primers. Samples were found to be infected by SCMV and SCSMV showed the expected amplicon size of 891bp and 690 respectively during RT-PCR.

Keywords

Prevalence, Mosaic, Incidence, RT-PCR. SCMV and SCSMV

Conclusion

The study serves as a comprehensive report to date on the mosaic disease incidence in sugarcane growing regions of Andhra Pradesh. The findings of this study shows that SCMV and SCSMV infections have occurred in sugarcane fields in Andhra Pradesh and has spread to new areas, primarily through infected setts, vectors and planting material. Some of the cultivars were found more susceptible to mosaic compared to others. This study emphasize the importance of SCMV and SCSMV indexing in planting material prior to distribution of seed setts to the farmers by the sugar industries of Andhra Pradesh which will ensure healthy planting material to the growers leading to higher sugarcane productivity and cane quality.

References

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How to cite this article

G. Vamsi Krishna, V. Manoj Kumar, P. Kishore Varma, B..Bhavani and G. Vijaya Kumar (2023). Prevalence and Detection of viruses Associated with Mosaic Disease of Sugarcane in Andhra Pradesh. Biological Forum – An International Journal, 15(5): 731-737.