Prevalence of Extended Spectrum Β-Lactamse Producing, Antibiotic Resistant Escherichia coli in Fresh Fish and Fish Waste in Retail Fish Markets of Navi Mumbai, India

Author: Niroli Roopa, Rathod Vandana, Talloli Narmada, Samruddhi Joshi and Dhananjay Desai

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Abstract

Transmission of antimicrobial genes through chain remain major public threat globally. Antibiotic susceptibility patterns of Escherichia coli were studied on fresh seafood found in retail market. ESBL E. coli isolated from 430 (fresh fish, fish waste and associated environment) fresh fish samples were examined in light of the phenotypic traits associated with antibiotic resistance. Of them, 60 ESBL positive E. coli were isolated. A large number of isolates were amoxicillin-resistant (98.4%), cefepime (91.9%), and both cefepime and ceftazidime (91.1%). Relatively higher susceptibilities were recorded against colistin (100%) cefotaxime-clavulanic acid and amoxyclav each with 85.5% respectively. All 60 isolates showed resistance to more than 03 antibiotics. Two isolates EC01 and EC59 showed resistance to 14 antibiotics. MIC values of 87% isolates were 1024μg/ml of cefotaxime. E-test showed resistance against multi enzyme MICTM strips for 20 isolates. The Multiple Antibiotic Resistance Index for the 60 isolates ranged from 0.2 to 0.87, showing that sources with a high risk of contamination had the highest levels of contamination. These studies reveal more prevalence of E. coli in fish waste samples than fresh fish. Thus, it draws attention to the threat of the spread of communities of seafood consumers who ingest multidrug resistant E. coli as well as the necessity of improving the sanitation of coastal waterways, retail markets, and landing areas. The present study gives direct contribution in monitoring antimicrobial gene in aquaculture value chain.

Keywords

Beta lactamase, E. coli, Seafood, MIC, Antibiotic Resistance

Conclusion

Studies on antimicrobial resistance in E. coli from food animals thereby contaminating humans has drawn special attention towards migration of resistance gene from animal to human through close association with animal reservoirs is limited and no such deep cohort studies in India are prevailing. Such studies are of urgent need due to the environment and public health concern. Pathogen resistance to new generation cephalosporins could present significant difficulties. To stop the spread of antibiotic resistance in coastal waters in such a scenario, efficient management measures must be employed. These studies therefore highlight the requirement for a thorough analysis of the application of antibiotics and growth promoters in the food and animal production chains.

References

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How to cite this article

Niroli Roopa, Rathod Vandana, Talloli Narmada, Samruddhi Joshi and Dhananjay Desai (2023). Prevalence of Extended Spectrum Β-Lactamse Producing, Antibiotic Resistant Escherichia coli in Fresh Fish and Fish Waste in Retail Fish Markets of Navi Mumbai, India. Biological Forum – An International Journal, 15(5): 1230-1237.