Probit Analysis for Lethality, Injury and Sterility in M1 Generation of MDU 1 Barnyard Millet (Echinochloa frumentacea)

Author: M. Karthikeyan, C. Vanniarajan*, M. Vetriventhan, A. Nirmalakumari, R. Shanmuga Sundaram, T. Sivakumar6 and G. Srinivasan

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Abstract

MDU 1 is the recently released barnyard millet variety from Agricultural College and Research Institute, TNAU, Madurai. The duration of this variety is about 90–95 days. As the crop is mostly raised under rainfed conditions, early duration (75–80 days) barnyard millet varieties are preferred. Therefore, the MDU 1 barnyard millet was treated with the chemical mutagens to obtain desirable mutants. Since chemical mutagenesis produces more point mutations, Ethyl Methane Sulphonate (EMS) and Sodium Azide (SA) were selected for this study. The variety MDU 1 Barnyard millet was subjected to treatments of 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM and 60 mM of both the mutagens EMS and SA. Probit analysis was made and LD50 dose for EMS was 16.19 mM and 14.14 mM for Sodium Azide. Other characters such as survival percentage, shoot length, root length, plant height at maturity, pollen fertility and seed fertility were assessed in addition to germination percentage. All the characters were found

Keywords

Barnyard millet, Ethyl Methane Sulphonate, Sodium Azide, LD50 value.