The folic acid is known as vitamin B9 which is converted into folate by body used as a dietary supplement and in food fortification as it is more stable during the processing and storage. Folic acids are used to treat or prevent folate deficiency anaemia, help baby’s brain development, shull and spinal cord develop properly in pregnancy, prevent liver and heart disease, to avoid development problems such as spina bifida etc. The source of folate is dark green leafy, vegetables, beans, peas, nuts, fermented vegetables, probiotic tablet, orange, lemon, bananas etc. The fermented product contains probiotic source which can produce folate. The study was conducted by using prebiotic sources like curd, cheese and Bifilac tablet. The microorganism isolated from prebiotic source could use as probiotics in controlling the harmfull microorganism and restoring the natural microflora inside the gastrointestinal tract due to folate production. Thus, the presence of the health beneficial microflora will give a positive impact when consume pathogenic microbes. PABA (Para amino benzoic acid) is necessary for the synthesis of folic acid. When the Para amino benzoic acid was supplemented in the peptone broth the bacteria strains can produce folic acid

pre-biotic, probiotic, lactic acid bacteria, PABA, folic acid

The folic acid is known as vitamin B9 which is converted into folate by body is used as a dietary supplement and in food fortification as it is more stable during the processing and storage. Folate helps the body make healthy red blood cells and is found in certain foods. Folic acids are used to treat or prevent folate deficiency anaemia, help baby’s brain development, shull and spinal cord develop properly in pregnancy, prevent liver and heart disease, to avoid development problems such as spina bifida etc. The source of folate is dark green leafy, vegetables, beans, peas, nuts, fermented vegetables, probiotic tablet, orange, lemon, bananas etc. The fermented product contains a probiotic source which can produce folate. The biochemical test like catalase test, motility, gas production test that indicates the bacterial isolates are Lactic acid producing bacteria. Microorganism isolated from prebiotic source could use as probiotics in controlling the harmful microorganism and restoring the natural microflora inside the gastrointestinal tract due to folate production. Thus, the presence of the health-beneficial microflora will give a positive impact when consuming pathogenic microbes, when the para-aminobenzoic acid was supplemented in the peptone broth, because PABA is necessary for the synthesis of folic acid (Rossi et al., 2011). The bacterial strain FA2 shows the highest growth in Fig. 9 with compare to the others in lactose peptone broth supplemented with PABA or without PABA

Madhusmita Konwar, Pranit Saikia and Jyotismita Konwar (2025). Production of Folic Acid using Folate-producing Bacteria from Different Prebiotic Sources. Biological Forum, 17(4): 60-64