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Protease Isoforms of Cotton Bollworm Pectinophora gossypiella: A Biochemical Insight

Author: Heena L. Nadaf, Ashwini K. Sirsat and Vandana K. Hivrale

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Abstract

The cotton bollworm larva, Pectinophora gossypiella is a threat to cotton crops globally. The pest attack result in qualitative and quantitative loss. Gut proteases of pest are studied to target its digestive system for inhibition. The biochemical and electrophoretic properties of proteases from the digestive system of Pectinophora gossypiella were determined. By using a well-diffusion assay, it was confirmed that P. gossypiella gut extract contained proteases. The proteolytic activity obtained in the solution assay was 0.116 Umg. The impact of varying pH and temperatures on proteolytic activity was assessed. At pH 10 and a temperature of 50°C, it showed that proteolytic activity was at its peak. To see the isoform of proteases in gel, native PAGE gelatine zymography was used. There are a total of 8 protease isoforms in all instars. The total protein profile of P. gossypiella was also determined. There is currently no information available about the digestive proteases of P. gossypiella. Detailed insights into the gut proteases of P. gossypiella can be used to create and enhance successful pest management techniques based on protease inhibitors.

Keywords

Pectinophora gossypiella, Pink bollworm, Proteases, protease isoforms, Gelatine-zymography

Conclusion

The presence of 8 protease isoforms suggests enormous dependency of P. gossypiella on proteases for food digestion. Any impairment in protein digestion can hamper the growth and survival of insects. The protease inhibitors are specific in action and it can inhibit to its complementary proteases only. In this view, to find a potent inhibitors against P. gossypiella, detailed knowledge of its gut proteases is required. This study provides biochemical characterization of P. gossypiella proteases along with detailed map of protease isoforms on gel.

References

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How to cite this article

Heena L. Nadaf, Ashwini K. Sirsat and Vandana K. Hivrale (2023). Protease Isoforms of Cotton Bollworm Pectinophora gossypiella: A Biochemical Insight. Biological Forum – An International Journal, 15(4): 33-39.