Proteases: Industrial Applications and Approaches used in Strain Improvement

Author: Neha Thakur, Meghna Goyal, Saweta Sharma and Dinesh Kumar

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Abstract

Proteolytic enzymes are the enzymes that bring about degradation of the proteins into peptides and amino acids. They are obtained from many sources such as plants, animals, micro-organisms. The enzymes isolated from micro-organisms are more preferred source and less costly as compare to the enzymes obtained from the plants and animals. Proteases are now widely used in pharmaceutical, leather, industrial waste management, brewing industry, food industry and providing economic benefits. Due to rapid increase in the applications of proteases there is continuous demand in both qualitative and quantitative enhancement of enzyme through strain improvement by inducing genetic variation. Enhanced production of enzymes and subsequent screening of parent and mutated strain through systemic mutagenesis is involved in strain improvement. Many physical and chemical mutagenic agents are generally used for yield improvement. This review will highlight the overview on classification, applications and

Keywords

Proteases, Classification, Sources, Physical and Chemical mutagenesis.

Conclusion

The demand of protease enzyme gaining day by day at commercial, industrial, pharmaceutical, analytical, diagnostic sectors. They occur ubiquitously in, animals, plants and microorganisms. As the enzymes obtained from the animal and plants are not much effective and costly so the enzymes produced with the help of micro-organisms are mostly preferred due to their rapid growth, and with limited space requirement for cultivation. Protease enzymes are utilised in food industries, fed industries, pharmaceutical industries, leather industries. Proteases are also a great source for management of waste. Their degradative nature also increases their useful for protein digestion in tissue dissociation, cell isolation and in cell culturing. Due to extensive use of protease enzyme at various industrial applications, strain improvement brings about hyper production of enzyme which ultimately leads to the reduced production cost of enzyme.

References

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How to cite this article

Neha Thakur, Meghna Goyal, Saweta Sharma and Dinesh Kumar (2018).Proteases: Industrial Applications and Approaches used in Strain Improvement Biological Forum – An International Journal 10(1): 158-167.